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复垦垃圾场中[具体植物 1]和[具体植物 2]冠层下土壤细菌群落的比较

Comparison of Soil Bacterial Communities under Canopies of and in a Reclaimed Waste Dump.

作者信息

Hou Huping, Liu Haiya, Xiong Jinting, Wang Chen, Zhang Shaoliang, Ding Zhongyi

机构信息

Engineering Research Center of Ministry of Education for Mine Ecological Restoration, China University of Mining and Technology, Xuzhou 221116, China.

School of Public Policy and Management, China University of Mining and Technology, Xuzhou 221116, China.

出版信息

Plants (Basel). 2023 Feb 20;12(4):974. doi: 10.3390/plants12040974.

Abstract

To compare the effects of different remediation tree species on soil bacterial communities and provide a theoretical basis for the selection of ecosystem function promotion strategies after vegetation restoration, the characteristic changes in soil bacterial communities after and reclamation were explored using high-throughput sequencing and molecular ecological network methods. The results showed that: (1) With the increase in reclamation years, the reclaimed soil properties were close to the control group, and the soil properties of were closer to the control group than those of . (2) The dominant bacteria under the canopies of and was the same. , , , , , , , and were the dominant bacteria in the restored soil, accounting for more than 95% of the total abundance. The average values of the Shannon diversity index, Simpson diversity index, Chao 1 richness estimator, and abundance-based coverage estimator of the bacterial community in the reclaimed soil were higher than those in the reclaimed soil. The influence of reclamation years on the bacterial community of samples is greater than that of species types. (3) The results of ecological network construction showed that the total number of nodes, total number of connections, and average connectivity of the soil bacterial network under reclamation were greater than those under reclamation. The bacterial molecular ecological network under was more abundant. (4) Among the dominant bacteria, the relative abundance of was negatively correlated with soil pH, soil total nitrogen content, and the activities of urease, invertase, and alkaline phosphatase, while the relative abundance of and was positively correlated with these environmental factors. The relationship between the soil bacterial community of and and the environmental factors is not completely the same, and even the interaction between some environmental factors and bacteria is opposite.

摘要

为比较不同修复树种对土壤细菌群落的影响,并为植被恢复后生态系统功能促进策略的选择提供理论依据,采用高通量测序和分子生态网络方法,探究了[具体树种1]和[具体树种2]复垦后土壤细菌群落的特征变化。结果表明:(1)随着复垦年限增加,复垦土壤性质接近对照组,且[具体树种1]复垦土壤性质比[具体树种2]复垦土壤性质更接近对照组。(2)[具体树种1]和[具体树种2]树冠下的优势细菌相同。[具体细菌种类1]、[具体细菌种类2]、[具体细菌种类3]、[具体细菌种类4]、[具体细菌种类5]、[具体细菌种类6]、[具体细菌种类7]和[具体细菌种类8]是恢复土壤中的优势细菌,占总丰度的95%以上。[具体树种1]复垦土壤中细菌群落的香农多样性指数、辛普森多样性指数、Chao 1丰富度估计器和基于丰度的覆盖率估计器的平均值高于[具体树种2]复垦土壤。复垦年限对样品细菌群落的影响大于树种类型。(3)生态网络构建结果表明,[具体树种1]复垦下土壤细菌网络的节点总数、连接总数和平均连通性大于[具体树种2]复垦下的。[具体树种1]下的细菌分子生态网络更丰富。(4)在优势细菌中,[具体细菌种类9]的相对丰度与土壤pH、土壤全氮含量以及脲酶、转化酶和碱性磷酸酶的活性呈负相关,而[具体细菌种类10]和[具体细菌种类11]的相对丰度与这些环境因素呈正相关。[具体树种1]和[具体树种2]的土壤细菌群落与环境因素的关系并不完全相同,甚至一些环境因素与细菌之间的相互作用是相反的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b21/9964797/8eec2969fce1/plants-12-00974-g001.jpg

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