Wang Jiawei, Yang Hong, Liu Xuyan, Wang Jiawei, Chang Jiang
Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology Beijing 100124 China
Beijing Drainage Group Co. Ltd Beijing 100022 China.
RSC Adv. 2020 Oct 7;10(61):37194-37201. doi: 10.1039/d0ra05908k.
To achieve the stable partial nitrification of municipal wastewater, activated sludge with high ammonia-oxidizing bacteria (AOB) content and low nitrite-oxidizing bacteria (NOB) content were immobilized in a polyvinyl alcohol filler. The effects of different levels of dissolved oxygen (DO) on the activity of AOB and NOB in the filler with temperature changes at the initial ammonia concentration of approximately 100 mg L were investigated. At 25 °C, when the DO concentration was greater than 5 mg L, the O-limiting condition inside the filler was destroyed as the demand for oxygen in AOB was certain, and resulted in enhanced NOB activity. At 15 °C, the DO concentration was not a key factor in determining the NOB activity due to the negative effect of temperature on NOB activity. The immobilized filler reactor of municipal wastewater achieved a nitrite accumulation rate (NAR) of >86.7 and >82% at 24-26 °C and 14-16 °C, respectively. Low temperatures did not deteriorate the stable partial nitrification performance. The total nitrogen (TN) removal efficiency of the immobilized filler reactor was 21.7-26.1% and 10.3-15.3% at 24-26 °C and 14-16 °C, respectively. The TN removal efficiency and NAR in municipal wastewater were higher as compared to simulated wastewater, indicating that the organic carbon in municipal wastewater enhanced nitrate reduction by denitrification. High-throughput sequencing analysis showed that denitrifying bacteria and nitrifying bacteria were identified as the predominant bacteria genera, while the dominant species of NOB was . This study is a viable approach to promoting partial nitrification in municipal WWTPs.
为实现城市污水的稳定部分硝化，将氨氧化细菌（AOB）含量高且亚硝酸盐氧化细菌（NOB）含量低的活性污泥固定在聚乙烯醇填料中。研究了在初始氨浓度约为100 mg/L时，不同溶解氧（DO）水平对填料中AOB和NOB活性随温度变化的影响。在25℃时，当DO浓度大于5 mg/L时，由于AOB对氧气的需求一定，填料内部的氧限制条件被破坏，导致NOB活性增强。在15℃时，由于温度对NOB活性有负面影响，DO浓度不是决定NOB活性的关键因素。城市污水固定化填料反应器在24 - 26℃和14 - 16℃时的亚硝酸盐积累率（NAR）分别>86.7%和>82%。低温并未降低稳定的部分硝化性能。固定化填料反应器在24 - 26℃和14 - 16℃时的总氮（TN）去除效率分别为21.7 - 26.1%和"10.3 - 15.3%。与模拟废水相比，城市污水中的TN去除效率和NAR更高，表明城市污水中的有机碳通过反硝化作用增强了硝酸盐还原。高通量测序分析表明，反硝化细菌和硝化细菌被鉴定为主要细菌属，而NOB的优势种为 。本研究是促进城市污水处理厂部分硝化的一种可行方法。
