Li Ping, Shi Rong Jiu, Zhao Feng, Yu Jing Hua, Cui Xiao Yang, Hu Jin Gui, Zhang Ying
Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Ying Yong Sheng Tai Xue Bao. 2019 Jan 20;30(1):95-107. doi: 10.13287/j.1001-9332.201901.010.
To reveal soil bacterial community structure and potential functions in larch forest during succession at Greater Khingan Mountains (Hanma National Nature Reserve), 16S rDNA was sequencing by Illumina Miseq. The results showed that the Proteobacteria, Acidobacteria, Verrucomicrobia, Bacteroidetes, Actinobacteria, Planctomycetes and Chloroflexi were the most dominant phyla in soils of larch forests at various successional stages. Along forest succession, Acidobacteria increased, while Chloroflexi decreased. Relative abundance of dominant phyla was different at various successional stages. The α diversity, Chao1, Shannon index and Simpson index of soil bacterial community had no significant difference among five succession stages, while significant differences in soil bacterial community structure were observed between young and medium larch, between young and over mature larch, and between near mature and mature larch. Bacterial community structure was mainly influenced by redox potential, pH and available phosphorus. The redox potential was the most important factor influencing soil bacterial community structure. Along the succession of larch forest, N-fixation, denitrification, ammonia oxidation and lignin breakdown decreased, dissimilatory sulfate reduction had down-up trend, carbon fixation had up-down trend, and alkaline phosphatase had no apparent trend. Bacterial community potential function was mainly influenced by redox potential and available phosphorus.
为揭示大兴安岭（汗马国家级自然保护区）落叶松林演替过程中土壤细菌群落结构及潜在功能，采用Illumina Miseq对16S rDNA进行测序。结果表明，变形菌门、酸杆菌门、疣微菌门、拟杆菌门、放线菌门、浮霉菌门和绿弯菌门是落叶松林不同演替阶段土壤中最主要的菌门。随着森林演替，酸杆菌门增加，而绿弯菌门减少。优势菌门的相对丰度在不同演替阶段有所不同。土壤细菌群落的α多样性、Chao1指数、香农指数和辛普森指数在五个演替阶段之间无显著差异，但在幼龄落叶松与中龄落叶松之间、幼龄落叶松与过熟落叶松之间以及近熟落叶松与成熟落叶松之间，土壤细菌群落结构存在显著差异。细菌群落结构主要受氧化还原电位、pH值和有效磷的影响。氧化还原电位是影响土壤细菌群落结构的最重要因素。随着落叶松林的演替，固氮、反硝化、氨氧化和木质素分解减少，异化硫酸盐还原呈先降后升趋势，碳固定呈先升后降趋势，碱性磷酸酶无明显趋势。细菌群落潜在功能主要受氧化还原电位和有效磷的影响。
