Sui Xin, Zhang Rong-Tao, Xu Nan, Liu Ying-Nan, Chai Chun-Rong, Wang Ji-Feng, Fu Xiao-Ling, Zhong Hai-Xiu, Ni Hong-Wei
College of Forestry, Northeast Forestry University, Harbin 150040, China.
National and Provincial Joint Engineering Laboratory of Wetlands and Ecological Conservation, Institute of Nature & Ecology, Heilongjiang Academy of Sciences, Harbin 150040, China.
Huan Jing Ke Xue. 2016 Sep 8;37(9):3598-3605. doi: 10.13227/j.hjkx.2016.09.043.
To understand the wetland soil fungal community structure and diversity in different degeneration wetlands, the topsoil (0-20) of three different degeneration wetlands were collected in the Sanjiang Plain field experiment station of the Institute of Nature and Ecology, Heilongjiang Academy of Sciences. The distribution and variation of soil fungal diversity were assessed by high-throughput sequencing method. The results showed that Shannon-Wiener index increased from marsh wetland marsh meadow wetland meadow wetland. Sequence blast showed that the fungal taxonomy belonged to Ascomycota, Basidiomycota, Chytridiomycota, Fungi_unclassified, Zygomycota, which dominant fungi were Fungi_unclassified (75.12%),Ascomycetes (56. 56%), Basidiomycetes (72.65%) in the three degeneration wetlands, respectively. The fungal structure compositions and diversities of marsh meadow wetland and meadow wetland were similar according to Heatmap analysis. The fungal community structure was influenced by soil nutrients (explained 88.62%) and plant composition (explained 9.85%) through the Variation partition analysis (VPA). In conclusion, the fungal community structure was significantly different, which was influenced by soil water content, in different degeneration wetlands in Sanjiang plain. The results may supply scientific basis for studying fungal diversity and spatial heterogeneity in degeneration wetlands.
为了解不同退化湿地中湿地土壤真菌群落结构和多样性，在黑龙江省科学院自然与生态研究所三江平原野外实验站采集了三种不同退化湿地的表层土壤（0 - 20厘米）。采用高通量测序方法评估土壤真菌多样性的分布和变化。结果表明，香农 - 维纳指数从沼泽湿地、沼泽化草甸湿地到草甸湿地呈增加趋势。序列比对显示，真菌分类属于子囊菌门、担子菌门、壶菌门、未分类真菌、接合菌门，在三种退化湿地中，优势真菌分别为未分类真菌（75.12%）、子囊菌（56.56%）、担子菌（72.65%）。根据热图分析，沼泽化草甸湿地和草甸湿地的真菌结构组成和多样性相似。通过变异分配分析（VPA）可知，真菌群落结构受土壤养分（解释率88.62%）和植物组成（解释率9.85%）的影响。总之，三江平原不同退化湿地的真菌群落结构存在显著差异，受土壤含水量影响。研究结果可为研究退化湿地真菌多样性和空间异质性提供科学依据。
