Wang Fan, Liu Kai, Lin Xing, Zhou Zheng, Li Xiang, Huang Yong
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Institute of Environmental Biotechnology, Suzhou University of Science and Technology, Suzhou 215009, China.
Huan Jing Ke Xue. 2017 Aug 8;38(8):3415-3421. doi: 10.13227/j.hjkx.201701185.
The effect of the TOC to NH-N ratio on nitrogen removal rate in an ANAMMOX sequencing batch reactor was studied for the short term and long term. Short-term results showed the maximum TOC/NH-N that ANAMMOX could afford was 1.4. At the same time, the rate of nitrogen removal was 0.26 kg·(m·d). The maximum nitrogen removal rate of 0.34 kg·(m·d) was obtained when TOC/NH-N was less than 0.4 in the long-term experiment. Whereas the nitrogen removal rate of the reactor decreased continuously when TOC/NH-N exceeded 0.4, and it was difficult to recover the activity of the ANAMMOX bacteria in the short term. qPCR results showed that the amount of ANAMMOX bacteria dropped from 2.9×10 copies·mL to 3.15×10 copies·mL with the increase of organics. When TOC/NH-N exceeded 1.6, NH-N could hardly be removed by the ANAMMOX bacteria, which were severely inhibited. The number of denitrifying bacteria was 3.0×10 copies·mL; meanwhile, most of the NO-N in the reactor was removed via denitrification.
研究了厌氧氨氧化序批式反应器中总有机碳（TOC）与氨氮（NH-N）的比例对短期和长期脱氮率的影响。短期结果表明，厌氧氨氧化能够承受的最大TOC/NH-N为1.4。同时，脱氮率为0.26 kg·(m·d)。长期实验中，当TOC/NH-N小于0.4时，获得了最大脱氮率0.34 kg·(m·d)。然而，当TOC/NH-N超过0.4时，反应器的脱氮率持续下降，且短期内厌氧氨氧化菌的活性难以恢复。定量聚合酶链反应（qPCR）结果表明，随着有机物含量的增加，厌氧氨氧化菌的数量从2.9×10 copies·mL降至3.15×10 copies·mL。当TOC/NH-N超过1.6时，厌氧氨氧化菌几乎无法去除NH-N，受到严重抑制。反硝化细菌的数量为3.0×10 copies·mL；同时，反应器中的大部分亚硝酸盐氮（NO-N）通过反硝化作用被去除。
