Zhang Cui-Jing, Shen Ju-Pei, Sun Yi-Fei, Wang Jun-Tao, Yang Zhong-Ling, Han Hong-Yan, Zhang Li-Mei, Wan Shi-Qiang, He Ji-Zheng
State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Huan Jing Ke Xue. 2017 Aug 8;38(8):3463-3472. doi: 10.13227/j.hjkx.201702178.
Soil ammonia oxidizers, as key players for the ammonia oxidation process in soil N cycling, could respond, adapt, and give feedback to global change. In this research, soil samples were collected from a long-term field experiment with increased precipitation and warming in a temperate steppe of Inner Mongolia. We analyzed the responses of the abundance, diversity, and community structure of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) to warming and increased precipitation using quantitative real-time PCR, terminal restriction fragment length polymorphism (T-RFLP), and clone library. The results showed that increased precipitation significantly stimulated soil pH and warming significantly reduced soil respiration (SR). No significant difference was detected regarding the abundances of genes across all treatments, whereas increased precipitation significantly affected the community structure of soil AOB. However, the interactive effect between warming and increased precipitation had no significant influence on the community structure of soil ammonia oxidizers. The result of the structural equation model indicated that the plant diversity and community structures of soil ammonia oxidizers were significantly correlated, suggesting that there were certain relationships among climate change, microbes, and plants. In conclusion, this study confirmed that soil microorganisms had the ability to adapt to climate change, which could provide important information for predicting future changes in ecosystems.
土壤氨氧化菌作为土壤氮循环中氨氧化过程的关键参与者，能够对全球变化做出响应、适应并反馈。本研究中，土壤样本采自内蒙古温带草原一个降水增加和温度升高的长期田间试验。我们使用定量实时PCR、末端限制性片段长度多态性（T-RFLP）和克隆文库分析了氨氧化古菌（AOA）和氨氧化细菌（AOB）的丰度、多样性及群落结构对温度升高和降水增加的响应。结果表明，降水增加显著提高了土壤pH值，温度升高显著降低了土壤呼吸（SR）。所有处理间基因丰度未检测到显著差异，而降水增加显著影响了土壤AOB的群落结构。然而，温度升高和降水增加的交互作用对土壤氨氧化菌的群落结构没有显著影响。结构方程模型结果表明，植物多样性与土壤氨氧化菌的群落结构显著相关，这表明气候变化、微生物和植物之间存在一定关系。总之，本研究证实土壤微生物具有适应气候变化的能力，这可为预测未来生态系统变化提供重要信息。
