降钙素和降钙素基因相关肽在培养的LLC-PK1肾细胞中与同一受体相互作用。

Calcitonin and calcitonin gene-related peptide interact with the same receptor in cultured LLC-PK1 kidney cells.

作者信息

Wohlwend A, Malmström K, Henke H, Murer H, Vassalli J D, Fischer J A

出版信息

Biochem Biophys Res Commun. 1985 Sep 16;131(2):537-42. doi: 10.1016/0006-291x(85)91269-0.

Abstract

Calcitonin and calcitonin gene-related peptide stimulate adenylate cyclase activity and plasminogen activator production in cultured renal tubular LLC-PK1 cells. Salmon [125I]calcitonin and human [125I]calcitonin gene-related peptide bound specifically to the cells. Salmon [125I]calcitonin binding was reduced at lower concentrations of non-radioactive salmon calcitonin than of human calcitonin gene-related peptide. For the stimulation of adenylate cyclase activity and plasminogen activator production, the potency of salmon calcitonin was higher than that of human calcitonin and calcitonin gene-related peptide. In a subclone of LLC-PK cells lacking salmon calcitonin binding sites, no specific binding of [125I]CGRP occurred, and adenylate cyclase activity and plasminogen activator production was not increased by the peptides. Thus, in LLC-PK1 cells the stimulation of adenylate cyclase activity and plasminogen activator production by calcitonin gene-related peptide is probably mediated by the calcitonin receptor.

摘要

降钙素和降钙素基因相关肽可刺激培养的肾小管LLC-PK1细胞中的腺苷酸环化酶活性并促进纤溶酶原激活物的产生。鲑鱼[125I]降钙素和人[125I]降钙素基因相关肽可特异性结合到这些细胞上。与非放射性人降钙素基因相关肽相比，较低浓度的非放射性鲑鱼降钙素即可降低鲑鱼[125I]降钙素的结合。对于腺苷酸环化酶活性的刺激和纤溶酶原激活物的产生，鲑鱼降钙素的效力高于人降钙素和降钙素基因相关肽。在缺乏鲑鱼降钙素结合位点的LLC-PK细胞亚克隆中，[125I]降钙素基因相关肽未发生特异性结合，并且这些肽也未增加腺苷酸环化酶活性和纤溶酶原激活物的产生。因此，在LLC-PK1细胞中，降钙素基因相关肽对腺苷酸环化酶活性的刺激和纤溶酶原激活物的产生可能是由降钙素受体介导的。