Tan Yang, Li Ji, Xu Qiao, Fu Lei, You Shi-Jie, Wang Shuo
Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environment and Civil Engineerin, Jiangnan University, Wuxi 214122, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Huan Jing Ke Xue. 2017 Jun 8;38(6):2486-2495. doi: 10.13227/j.hjkx.201610110.
In order to investigate the effects of temperature and organic loading on the bioactivity reactivation of anoxic biofilm in suspended carriers, the experiment was carried out under low organic loading, shock loading and low temperature conditions. The experimental results showed that the increase of polysaccharide in extracellular polymeric substance (EPS) was important during bioactivity reactivation process, and polysaccharide content was higher than that of protein under shock loading and low temperature conditions, and the contents of both polysaccharide and protein were much higher under low organic loading condition. Anoxic biofilm achieved excellent applicability in shock loading condition, moreover anoxic biofilm reactivated under low organic loading had lowest denitrication capacity and anoxic biofilm recovered at low temperature was in between. In addition, the relative abundance of Proteobacteria under low organic loading, shock loading and low temperature conditions increased from 72.23%, 78.66% and 76.35% to 83.17%, 84.30% and 80.46% at the end of the reactivation process (25th day), respectively. However, the relative abundance of Proteobacteria under low organic loading was lower than those of other reactors, indicating that the anoxic suspended carriers were less suitable for total nitrogen removal in wastewater treatment plant. The increase in relative abundance of was significant for enhancing total nitrogen removal, and anoxic biofilm possessed comparatively rich biodiversity when the denitrification capability was recovered. The presence of could promote the denitrification capacity of biofilm at low temperature.
为了研究温度和有机负荷对悬浮载体中缺氧生物膜生物活性恢复的影响，在低有机负荷、冲击负荷和低温条件下进行了实验。实验结果表明，胞外聚合物（EPS）中多糖的增加在生物活性恢复过程中很重要，在冲击负荷和低温条件下多糖含量高于蛋白质，而在低有机负荷条件下多糖和蛋白质含量均更高。缺氧生物膜在冲击负荷条件下具有良好的适用性，此外，在低有机负荷下恢复活性的缺氧生物膜脱氮能力最低，在低温下恢复的缺氧生物膜脱氮能力介于两者之间。此外，在低有机负荷、冲击负荷和低温条件下，变形菌门的相对丰度在恢复过程结束时（第25天）分别从72.23%、78.66%和76.35%增加到83.17%、84.30%和80.46%。然而，低有机负荷下变形菌门的相对丰度低于其他反应器，这表明缺氧悬浮载体不太适合污水处理厂的总氮去除。 相对丰度的增加对提高总氮去除具有重要意义，并且当脱氮能力恢复时，缺氧生物膜具有相对丰富的生物多样性。 的存在可以促进低温下生物膜的脱氮能力。
