Moss M, Norris J S, Peck E J, Schwartz R J
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
Exp Cell Res. 1978 May;113(2):445-50. doi: 10.1016/0014-4827(78)90388-9.
Lactoperoxidase catalysed iodination was used to label surface proteins of chick embryo muscle cells during myogenesis. Both quantitative and qualitative changes were observed between 125I-labelled surface proteins of pre-fusion, mid-fusion, and post-fusion cells. Significantly, two bands at 245,000 molecular weight were present at pre-fusion but were observed as a single band at mid- and post-fusion. Radioactivity in this band increased selectively at post-fusion with a concomitant increase in lower molecular weight labelled proteins.
在肌生成过程中，利用乳过氧化物酶催化碘化作用来标记鸡胚肌细胞的表面蛋白。在预融合、融合中期和融合后期细胞的¹²⁵I标记表面蛋白之间观察到了定量和定性的变化。值得注意的是，分子量为245,000的两条带在预融合时存在，但在融合中期和后期观察到为一条带。该条带中的放射性在融合后期选择性增加，同时较低分子量的标记蛋白也增加。
