体外骨骼肌肌生成过程中质膜蛋白的生物合成
Biosynthesis of plasma-membrane proteins during myogenesis of skeletal muscle in vitro.
作者信息
Cates G A, Holland P C
出版信息
Biochem J. 1978 Sep 15;174(3):873-81. doi: 10.1042/bj1740873.
Abstract
- Surface labelling of plasma-membrane proteins with 125I, catalysed by lactoperoxidase, and radioactive l-fucose incorporation into glycoprotein were used as plasma-membrane markers for skeletal-muscle cells in culture. 2. Plasma membranes were prepared at various stages of myogenesis in vitro and rates of synthesis and accumulation of proteins in the membranes were compared. 3. Increased synthesis and accumulation of a protein of apparent mol.wt. 70000 occurred in the plasma-membrane fraction concomitant with the onset of myoblast fusion. 4. In cultures in which fusion of myoblasts was inhibited by 5'-bromo-2-deoxyuridine, synthesis and accumulation of the protein of apparent mol.wt. 70000 was selectively inhibited. 5. It is suggested the protein of apparent mol.wt. 70000 may be involved in the process of myoblast fusion.
摘要
- 用乳过氧化物酶催化，对质膜蛋白进行¹²⁵I表面标记，以及将放射性L-岩藻糖掺入糖蛋白，被用作培养的骨骼肌细胞质膜标记物。2. 在体外肌生成的不同阶段制备质膜，并比较膜中蛋白质的合成和积累速率。3. 随着成肌细胞融合的开始，质膜部分中一种表观分子量为70000的蛋白质的合成和积累增加。4. 在成肌细胞融合被5'-溴-2-脱氧尿苷抑制的培养物中，表观分子量为70000的蛋白质的合成和积累被选择性抑制。5. 提示表观分子量为70000的蛋白质可能参与成肌细胞融合过程。
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