Department of Physiology and Pharmacology, SUNY Downstate Medical Center, Brooklyn, New York, USA.
Department of Medicine, Division of Hematology/Oncology, SUNY Downstate Medical Center, Brooklyn, New York, USA.
J Investig Med. 2018 Oct;66(7):1055-1063. doi: 10.1136/jim-2018-000717. Epub 2018 Jul 2.
Regulatory brain cytoplasmic 200 RNA (BC200 RNA) is highly expressed in human mammary carcinoma cells. Here, we ask whether BC200 RNA becomes detectable in peripheral blood of patients with invasive breast cancer. Using quantitative reverse-transcription PCR (qRT-PCR) methodology, we observed that BC200 RNA blood levels were significantly elevated, in comparison with healthy subjects, in patients with invasive breast cancer prior to tumorectomy (p=0.001) and in patients with metastatic breast cancer (p=0.003). In patients with invasive breast cancer who had recently undergone tumorectomy, BC200 RNA blood levels were not distinguishable from levels in healthy subjects. However, normality analysis revealed a heterogeneous distribution of patients in this group, including a subgroup of individuals with high residual BC200 RNA blood levels. In blood from patients with invasive breast cancer, BC200 RNA was specifically detected in the mononuclear leukocyte fraction. The qRT-PCR approach is sensitive enough to detect as few as three BC200 RNA-expressing tumor cells. Our work establishes the potential of BC200 RNA detection in blood to serve as a molecular indicator of invasive breast malignancy.
调控性脑细胞质 200 RNA（BC200 RNA）在人乳腺癌细胞中高度表达。在这里，我们探讨了 BC200 RNA 是否可在浸润性乳腺癌患者的外周血中检测到。我们采用定量逆转录 PCR（qRT-PCR）方法，观察到在接受肿瘤切除术之前的浸润性乳腺癌患者（p=0.001）和转移性乳腺癌患者（p=0.003）中，BC200 RNA 血液水平与健康受试者相比显著升高。在最近接受肿瘤切除术的浸润性乳腺癌患者中，BC200 RNA 血液水平与健康受试者无差异。然而，正态性分析显示该组患者的分布存在异质性，包括一组 BC200 RNA 血液水平较高的个体。在浸润性乳腺癌患者的血液中，可特异性检测到单核白细胞中存在 BC200 RNA。qRT-PCR 方法的灵敏度足以检测到少至三个表达 BC200 RNA 的肿瘤细胞。我们的工作确立了在血液中检测 BC200 RNA 作为浸润性乳腺癌恶性肿瘤分子标志物的潜力。
