Edwards P M, Verhaagen J, Spierings T, Schotman P, Jennekens F G, Gispen W H
Brain Res Bull. 1985 Sep;15(3):267-72. doi: 10.1016/0361-9230(85)90149-2.
The effects of ACTH4-10, a peptide fragment of corticotropin, on rat dorsal root ganglia (DRG), spinal cord and sciatic nerve were studied following a crush lesion of the sciatic nerve. The in vitro total protein synthesis rate of DRG L4, L5 and L6, measured one and three days after ipsilateral nerve crush, were not altered by various ACTH4-10 treatment regimes. Likewise, neither ACTH4-10 treatment of sham-operated rats nor in vitro exposure of control ganglia to peptide, resulted in changes in synthesis rate. Four days after crush lesion, the amounts of actin and tubulin in the ventral horn L2-L5 region of the spinal cord and of actin in DRG L5 were estimated following 2-dimensional separation. No significant effect of ACTH treatment was found. Degeneration-associated changes in the protein profiles of segments of sciatic nerve were not altered by ACTH4-10 treatment. The data are discussed in relation to the possible site of action of neurotrophic ACTH-like peptides.
在坐骨神经挤压损伤后，研究了促肾上腺皮质激素的肽片段促肾上腺皮质激素4-10（ACTH4-10）对大鼠背根神经节（DRG）、脊髓和坐骨神经的影响。在同侧神经挤压后1天和3天测量的DRG L4、L5和L6的体外总蛋白合成率，并未因各种ACTH4-10治疗方案而改变。同样，对假手术大鼠进行ACTH4-10治疗以及将对照神经节在体外暴露于该肽，均未导致合成率发生变化。挤压损伤4天后，在二维分离后估计脊髓腹角L2-L5区域的肌动蛋白和微管蛋白量以及DRG L5中的肌动蛋白量。未发现ACTH治疗有显著效果。ACTH4-10治疗未改变坐骨神经节段蛋白质谱中与变性相关的变化。结合神经营养性ACTH样肽可能的作用位点对数据进行了讨论。
