Bijlsma W A, Schotman P, Jennekens F G, Gispen W H
Neurosci Lett. 1983 Aug 8;38(3):297-302. doi: 10.1016/0304-3940(83)90385-3.
To measure protein synthesis at the level of the cell bodies of motoneurons of the sciatic nerve, anterior horns were punched out of the spinal cord using microdissection techniques and a cell-free system was prepared. Injections of ACTH1-16 and ACTH4-10 were shown to enhance protein synthesis in the cell-free system, 1.5 h after treatment. Neither crush lesion of the sciatic nerve nor systemic treatment with ACTH4-10 produced clear changes in overall protein synthesis. Thus, the increase in overall protein synthesis by acute treatment with ACTH-like peptides cannot explain the previously reported stimulation of axonal regeneration after nerve crush.
为了在坐骨神经运动神经元的细胞体水平测量蛋白质合成，使用显微解剖技术从脊髓中取出前角，并制备了无细胞系统。结果显示，在处理1.5小时后，注射促肾上腺皮质激素1 - 16和促肾上腺皮质激素4 - 10可增强无细胞系统中的蛋白质合成。坐骨神经挤压损伤和促肾上腺皮质激素4 - 10全身治疗均未使总蛋白质合成产生明显变化。因此，急性给予促肾上腺皮质激素样肽导致的总蛋白质合成增加并不能解释先前报道的神经挤压后轴突再生的刺激现象。
