Chua D Y, Pang M W, Rauff A, Aw S E, Chan S H
Cancer. 1985 Nov 1;56(9):2228-34. doi: 10.1002/1097-0142(19851101)56:9<2228::aid-cncr2820560916>3.0.co;2-w.
Cancer of the breast is the most common tumor in females in Singapore, with the rate of 20.7 per 100,000 per year (1977 estimate), which is predicted to increase to 29.8 per 100,000 women per year by 1995. A detailed histopathologic review of 50 primary breast cancer tumors analyzed for estrogen receptor (ER) level was carried out and a variety of morphologic features correlated with ER results to identify any factors that will improve the management and prognosis for breast cancer. Cytosol was incubated with 3H-estradiol in the presence and absence of cold diethylstilbestrol, and bound and free hormone were separated by Dextran-coated charcoal method. Tumors binding more than 5 fmol/mg cytosol protein were classified as ER-positive. Progesterone receptor (PR) level was analyzed in some specimens with the use of a similar method. Most of the patients were Chinese (90%). Three patients were Malays, one was Indian, and one was European in this series. Results indicated that there was strong correlation between ER level, age, and histologic grade of the tumors. No correlation existed between absence or presence of lymph node metastases and ER. Although there was a trend for ER-positive tumors to have a low-grade lymphocytic infiltration, the difference was not statistically significant.
乳腺癌是新加坡女性中最常见的肿瘤，发病率为每年每10万人中有20.7例（1977年估计数），预计到1995年将增至每年每10万女性中有29.8例。对50例原发性乳腺癌肿瘤进行了详细的组织病理学检查，分析其雌激素受体（ER）水平，并将各种形态学特征与ER结果相关联，以确定任何可改善乳腺癌治疗和预后的因素。在有和没有冷己烯雌酚存在的情况下，将胞质溶胶与3H-雌二醇一起孵育，并用葡聚糖包被的活性炭法分离结合型和游离型激素。与超过5 fmol/mg胞质溶胶蛋白结合的肿瘤被分类为ER阳性。使用类似方法在一些标本中分析了孕激素受体（PR）水平。大多数患者是华人（90%）。本系列中有3名患者是马来人，1名是印度人，1名是欧洲人。结果表明，ER水平、年龄和肿瘤组织学分级之间存在强相关性。有无淋巴结转移与ER之间无相关性。虽然ER阳性肿瘤有低度淋巴细胞浸润的趋势，但差异无统计学意义。
