Gao Man, Wang Kuo, Yang Mingyue, Meng Fanzheng, Lu Ruihua, Zhuang Huadong, Cheng Genhong, Wang Xiaosong
Department of Pediatrics, The First Hospital of Jilin University, Changchun, China.
Institute of Translational Medicine, The First Hospital of Jilin University, Changchun, China.
Front Immunol. 2018 Jun 18;9:1403. doi: 10.3389/fimmu.2018.01403. eCollection 2018.
pneumonia (MPP) is one of the most common community-acquired pneumonia; this study is to explore the immune-pathogenesis of children MPP.
Next-generation transcriptome sequencing was performed on the bronchoalveolar lavage fluid cells from six children with MPP and three children with foreign body aspiration as control. Some of the results had been validated by quantitative real-time PCR in an expanded group of children.
Results revealed 810 differentially expressed genes in MPP group comparing to control group, of which 412 genes including and were upregulated. These upregulated genes were mainly enriched in mononuclear cell proliferation and signaling biological processes. Kyoto encyclopedia of genes and genomes pathway analysis revealed that hematopoietic cell linage pathway, natural killer cell-mediated cytotoxicity pathway, and T cell receptor signaling pathway were significantly upregulated in MPP children. In addition, significant alternative splicing events were found in and genes, which may cause the differential expressions of these genes.
Our results suggest that NK and CD8+ T cells are over activated and proliferated in MPP children; the upregulated , and may play important roles in the pathogenesis of children MPP.
支原体肺炎（MPP）是最常见的社区获得性肺炎之一；本研究旨在探讨儿童MPP的免疫发病机制。
对6例MPP患儿和3例异物吸入患儿的支气管肺泡灌洗细胞进行二代转录组测序。部分结果在扩大的儿童组中通过定量实时PCR进行了验证。
结果显示，与对照组相比，MPP组有810个差异表达基因，其中包括 和 在内的412个基因上调。这些上调基因主要富集于单核细胞增殖和信号生物学过程。京都基因与基因组百科全书通路分析显示，造血细胞谱系通路、自然杀伤细胞介导的细胞毒性通路和T细胞受体信号通路在MPP患儿中显著上调。此外，在 和 基因中发现了显著的可变剪接事件，这可能导致这些基因的差异表达。
我们的结果表明，MPP患儿中NK细胞和CD8 + T细胞过度活化和增殖；上调的 、 和 可能在儿童MPP的发病机制中起重要作用。
