Moradi Mohammadreza, Saidijam Massoud, Yadegarazari Reza, Jahangard Leila, Seifi Maryam, Matinnia Nasrin, Ghaleiha Ali
Molecular Medicine Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Behavioral Disorders and Substance Abuse Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Basic Clin Neurosci. 2018 Mar-Apr;9(2):129-134. doi: 10.29252/NIRP.BCN.9.2.129.
According to the cumulative evidence, genes encoding GABA receptors inhibit neurotransmitters in CNS and are intricately involved in the pathogenesis of mood disorders. Based on this hypothesis, these genes may be expressed in bipolar patients. As a result, we evaluated the gene expressions of GABA-β3 and HT1D receptors to assess their associations with bipolar mood disorder.
In this study, 22 patients with bipolar I disorder (single manic episode) and 22 healthy individuals were enrolled. All participants were older than 15 years and had referred to Farshchian Hospital, Hamadan, Iran. They were diagnosed based on DSM IV-TR criteria and young mania rating scale in order to determine the severity of mania by a psychiatrist as bipolar Type 1 disorder in manic episode. We evaluated the expression of GABA-β3 and HT1D receptor genes in peripheral blood mononuclear cells, using real-time RT-PCR analysis.
In our study, a reduction in the gene expression of GABA-β3 and HT1D receptors was observed in peripheral blood mononuclear cells of the patients with bipolar disorders compared to the healthy controls.
The results of this study supports the hypothesis that the gene expression for serotonin and GABA receptors can be employed in elucidating the pathogenesis of bipolar disorders.
根据累积证据，编码γ-氨基丁酸（GABA）受体的基因在中枢神经系统中抑制神经递质，并与情绪障碍的发病机制密切相关。基于这一假设，这些基因可能在双相情感障碍患者中表达。因此，我们评估了GABA-β3和5-羟色胺受体1D（HT1D）的基因表达，以评估它们与双相情感障碍的关联。
在本研究中，招募了22例双相I型障碍（单次躁狂发作）患者和22名健康个体。所有参与者年龄均超过15岁，均来自伊朗哈马丹的法什奇安医院。他们根据《精神疾病诊断与统计手册》第四版修订版（DSM IV-TR）标准和青年躁狂评定量表进行诊断，以便由精神科医生确定躁狂发作时双相I型障碍的严重程度。我们使用实时逆转录-聚合酶链反应（RT-PCR）分析评估外周血单个核细胞中GABA-β3和HT1D受体基因的表达。
在我们的研究中，与健康对照组相比，双相情感障碍患者外周血单个核细胞中GABA-β3和HT1D受体的基因表达降低。
本研究结果支持以下假设，即血清素和GABA受体的基因表达可用于阐明双相情感障碍的发病机制。
