Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Biomaterials Group, Department of Nanotechnology and Advanced Materials, Materials & Energy Research Center, Karaj, Iran.
Curr Stem Cell Res Ther. 2019;14(2):196-210. doi: 10.2174/1574888X13666180703141956.
Bioactive glasses with different compositions have been extensively used as bone tissue engineering. Preparation, development and characterization of alginate pastes containing bioglass for bone repair applications were the purposes of this study.
The injectable bone pastes were produced from sol-gel derived bioactive glass nanoparticles with various CaO/PO ratios of 19, 9.5 and 4.75 and sodium alginate solutions with different concentrations of 1, 2 and 4 wt.%. The effect of CaO/PO and powder to liquid (P/L) ratios and alginate concentration on injectability, biodegradation, rheological properties, bioactivity and cellular behavior of the pastes have been studied. The behavior of human mesenchymal stem cells (hMSCs) in the presence of the pastes was assessed by MTT assay, biomineralization assay, ALP activity, Acridine orange staining and Alizarin red staining tests.
By adding sodium alginate, the pastes exhibited a thixotropy behavior. The storage modulus of all pastes was larger than the loss modulus in the frequency range of 0.1-100 s-1. Cytotoxicity evaluation results revealed that there was a critical amount of bioactive glass in pastes which are above the limit; the viability of hMSCs will be at risk. The pastes made of bioactive glass nanoparticles with CaO/PO = 9.5 and sodium alginate 1% with P/L ratio of 0.8 showed optimum behavior in terms of mineral carrying capacity, injectability characteristics, accellular bioactivity in SBF, loss weight and wash out behavior, proliferation and differentiation of hMSCs.
According to the results, the pastes prepared with sodium alginate solution and bioactive glass nanoparticles can be beneficial in bone tissue engineering.
具有不同成分的生物活性玻璃已被广泛用作骨组织工程材料。本研究旨在制备、开发和表征用于骨修复应用的含生物玻璃的藻酸盐糊剂。
本研究采用不同 CaO/PO 比（19、9.5 和 4.75）的溶胶-凝胶衍生的生物活性玻璃纳米粒子和不同浓度（1、2 和 4wt.%）的海藻酸钠溶液制备可注射骨糊剂。研究了 CaO/PO 比、粉末与液体（P/L）比和海藻酸钠浓度对糊剂可注射性、生物降解性、流变性能、生物活性和细胞行为的影响。通过 MTT 测定、生物矿化测定、碱性磷酸酶（ALP）活性测定、吖啶橙染色和茜素红染色试验评估了糊剂中存在的人骨髓间充质干细胞（hMSCs）的行为。
通过添加海藻酸钠，糊剂表现出触变性。在 0.1-100s-1 的频率范围内，所有糊剂的储能模量均大于损耗模量。细胞毒性评价结果表明，糊剂中存在一个临界的生物活性玻璃量，超过这个量，hMSCs 的存活率将受到威胁。在 CaO/PO=9.5 的生物活性玻璃纳米粒子和 1%的海藻酸钠（P/L 比为 0.8）组成的糊剂中，具有最佳的载矿能力、可注射性、SBF 中的无细胞生物活性、失重和洗脱行为、hMSCs 的增殖和分化。
根据研究结果，用海藻酸钠溶液和生物活性玻璃纳米粒子制备的糊剂可用于骨组织工程。
