Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
Int J Biol Macromol. 2019 Sep 15;137:1256-1267. doi: 10.1016/j.ijbiomac.2019.06.182. Epub 2019 Jul 4.
Past researches on bone regeneration field have shown the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition. However, there is no dedicated work on the effect of the aforementioned bio-glass on the polymer matrix composites. The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks. The prepared scaffolds were characterized by SEM, ATR-FTIR and XRD analysis. Compression strength of obtained highly porous composite scaffolds was remarkably enhanced by the presence of bio-glass particles. The maximum compressive strength (1.7 MPa) was obtained for alginate composite containing 1 g Mg-Zn-BG. In vitro evaluation such as swelling, bio-mineralization, biodegradation were carried out, which indicates that incorporation of bio-glass promotes apatite deposition on composite scaffolds. Cytotoxicity, cell attachment and proliferation and osteogenic differentiation were also evaluated by culturing MG-63 cells on scaffolds. ICP analysis were conducted after 60 days of incubation in PBS solution to verify the ion release capability of the composite scaffolds, particularly Zn and Mg ions, which resulted in significant antibacterial efficacy enhancement of composite scaffolds against E. coli and S. aureus bacteria.
过去的骨再生领域研究表明，生物活性玻璃组成中锌离子和镁离子的存在具有积极影响。然而，目前还没有专门针对上述生物玻璃对聚合物基质复合材料影响的研究工作。该方法的主要思路是通过将含有锌和镁的生物活性玻璃掺入海藻酸钠网络中，来提高骨复合支架的抗菌效果、生物活性和机械性能。通过 SEM、ATR-FTIR 和 XRD 分析对制备的支架进行了表征。生物玻璃颗粒的存在显著提高了获得的高多孔复合支架的压缩强度。在含有 1g Mg-Zn-BG 的海藻酸钠复合材料中获得了最大的压缩强度（1.7MPa）。进行了体外评价，如溶胀、生物矿化、生物降解，这表明生物玻璃的掺入促进了复合支架上磷灰石的沉积。还通过在支架上培养 MG-63 细胞来评估细胞毒性、细胞附着和增殖以及成骨分化。在 PBS 溶液中孵育 60 天后进行 ICP 分析，以验证复合支架的离子释放能力，特别是 Zn 和 Mg 离子，这导致了复合支架对大肠杆菌和金黄色葡萄球菌的显著抗菌效果增强。
