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牙周膜干细胞来源的外泌体与水凝胶促进牙槽骨缺损骨再生的实验研究

The Experimental Study of Periodontal Ligament Stem Cells Derived Exosomes with Hydrogel Accelerating Bone Regeneration on Alveolar Bone Defect.

作者信息

Zhao Yang, Gong Yujia, Liu Xianbo, He Jia, Zheng Bowen, Liu Yi

机构信息

Department of Orthodontics, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang 110002, China.

出版信息

Pharmaceutics. 2022 Oct 14;14(10):2189. doi: 10.3390/pharmaceutics14102189.

DOI:10.3390/pharmaceutics14102189
PMID:36297624
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9611133/
Abstract

INTRODUCTION

this study was conducted to investigate the osteogenic ability of periodontal ligament stem cells (PDLSCs) derived exosomes (PDLSCs-Exos) and the effect of PDLSCs-Exos with hydrogel on alveolar bone defect repairment in the rat.

METHODS

the PDLSCs were obtained through primary cell culture, and PDLSCs-Exos were purified by the ultracentrifugation method. The CCK-8 kit and ALP staining were used to explore the effect of PDLSCs-Exos on promoting the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). In vivo, the alveolar bone defect models were made mesial to the bilateral maxillary first molars of rats. MicroCT, HE staining, and Masson staining were used to analyze the new bone at the bone defect of rats.

RESULTS

the periodontal ligament stem cells and the periodontal ligament stem cells derived exosomes were successfully extracted. The results of the CCK-8 kit and ALP staining showed PDLSCs-Exos significantly promoted the proliferation osteogenic differentiation of BMSCs. In vivo experiment results revealed that compared with the control group and the hydrogel group, the rats in the hydrogel with exosomes group showed more new bone formation in alveolar bone defects.

CONCLUSION

Periodontal ligament stem cells and exosomes derived from periodontal ligament stem cells were successfully extracted. The results demonstrated that the hydrogel successfully delivered periodontal ligament stem cells derived exosomes for repairing alveolar bone defects in rats in vivo at the initial stage.

摘要

引言

本研究旨在探讨牙周膜干细胞（PDLSCs）来源的外泌体（PDLSCs-Exos）的成骨能力以及PDLSCs-Exos与水凝胶对大鼠牙槽骨缺损修复的影响。

方法

通过原代细胞培养获得PDLSCs，并采用超速离心法纯化PDLSCs-Exos。使用CCK-8试剂盒和碱性磷酸酶（ALP）染色来探究PDLSCs-Exos对促进骨髓间充质干细胞（BMSCs）增殖和成骨分化的作用。在体内，于大鼠双侧上颌第一磨牙近中制作牙槽骨缺损模型。采用显微CT、苏木精-伊红（HE）染色和马松（Masson）染色分析大鼠骨缺损处的新骨情况。

结果

成功提取了牙周膜干细胞及其来源的外泌体。CCK-8试剂盒和ALP染色结果显示，PDLSCs-Exos显著促进了BMSCs的增殖和成骨分化。体内实验结果表明，与对照组和水凝胶组相比，外泌体水凝胶组大鼠牙槽骨缺损处有更多新骨形成。

结论

成功提取了牙周膜干细胞及其来源的外泌体。结果表明，水凝胶在初期成功递送了牙周膜干细胞来源的外泌体用于修复大鼠体内的牙槽骨缺损。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75e4/9611133/45e739df6e45/pharmaceutics-14-02189-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75e4/9611133/b8c1a1192cfe/pharmaceutics-14-02189-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75e4/9611133/411a4f15595f/pharmaceutics-14-02189-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75e4/9611133/4fcfcf7b2cef/pharmaceutics-14-02189-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75e4/9611133/04360189fae8/pharmaceutics-14-02189-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75e4/9611133/d0d804976df7/pharmaceutics-14-02189-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75e4/9611133/45e739df6e45/pharmaceutics-14-02189-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75e4/9611133/b8c1a1192cfe/pharmaceutics-14-02189-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75e4/9611133/411a4f15595f/pharmaceutics-14-02189-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75e4/9611133/4fcfcf7b2cef/pharmaceutics-14-02189-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75e4/9611133/04360189fae8/pharmaceutics-14-02189-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75e4/9611133/d0d804976df7/pharmaceutics-14-02189-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75e4/9611133/45e739df6e45/pharmaceutics-14-02189-g006.jpg

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2
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4
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