Department of Physics, North Carolina State University, Raleigh, NC, USA.
Department of Chemistry, Osaka University, Osaka, 560-0043, Japan.
Sci Rep. 2018 Jul 3;8(1):10036. doi: 10.1038/s41598-018-28278-0.
We report that long double-stranded DNA confined to quasi-1D nanochannels undergoes superdiffusive motion under the action of the enzyme T4 DNA ligase in the presence of necessary co-factors. Inside the confined environment of the nanochannel, double-stranded DNA molecules stretch out due to self-avoiding interactions. In absence of a catalytically active enzyme, we see classical diffusion of the center of mass. However, cooperative interactions of proteins with the DNA can lead to directed motion of DNA molecules inside the nanochannel. Here we show directed motion in this configuration for three different proteins (T4 DNA ligase, MutS, E. coli DNA ligase) in the presence of their energetic co-factors (ATP, NAD).
我们报告说，在必要的辅因子存在下，在 T4 DNA 连接酶的作用下，限制在准 1D 纳米通道中的长双链 DNA 会发生超扩散运动。在纳米通道的受限环境中，由于自回避相互作用，双链 DNA 分子会伸展。在没有催化活性酶的情况下，我们看到了质心的经典扩散。然而，蛋白质与 DNA 的协同相互作用可以导致 DNA 分子在纳米通道内的定向运动。在这里，我们在存在能量辅因子（ATP、NAD）的情况下，对于三种不同的蛋白质（T4 DNA 连接酶、MutS、大肠杆菌 DNA 连接酶），在这种构象下显示出定向运动。
