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Identification of proteins involved in the regulation of yeast iso- 1-cytochrome C expression by oxygen.

作者信息

Arcangioli B, Lescure B

出版信息

EMBO J. 1985 Oct;4(10):2627-33. doi: 10.1002/j.1460-2075.1985.tb03980.x.

DOI:10.1002/j.1460-2075.1985.tb03980.x
PMID:2996882
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554553/
Abstract

On the basis of a gel electrophoresis retardation assay, protein(s) which interact specifically with the upstream activating site (UASc) of the yeast iso-1-cytochrome C (CYC1) gene were identified and separated by heparin ultrogel chromatography. DNase I protection experiments indicate that these factors protect a 23-bp sequence overlapping the UASc site previously defined. The specific binding activity is strongly reduced in extracts prepared from a wild-type strain grown anaerobically. It is absent in a mutant strain blocked in the biosynthesis of heme but it is restored upon the addition of the missing precursor, delta amino levulinic acid (dALA) to the growth medium. In contrast, the binding activity does not differ significantly in extracts form a wild-type strain grown in either glucose or glycerol as carbon source. These data strongly argue that the CYC1 UAS binding protein(s) that we have identified mediate the oxygen and heme control of cytochrome C biosynthesis.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcb9/554553/44ea3164ff5f/emboj00275-0215-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcb9/554553/874b406b5e40/emboj00275-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcb9/554553/a9e29e0e06c5/emboj00275-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcb9/554553/e0e3204a4250/emboj00275-0212-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcb9/554553/166f6042e7ce/emboj00275-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcb9/554553/b6eb23f7445b/emboj00275-0214-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcb9/554553/44ea3164ff5f/emboj00275-0215-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcb9/554553/874b406b5e40/emboj00275-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcb9/554553/a9e29e0e06c5/emboj00275-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcb9/554553/e0e3204a4250/emboj00275-0212-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcb9/554553/166f6042e7ce/emboj00275-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcb9/554553/b6eb23f7445b/emboj00275-0214-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcb9/554553/44ea3164ff5f/emboj00275-0215-a.jpg

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Identification of proteins involved in the regulation of yeast iso- 1-cytochrome C expression by oxygen.
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2
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本文引用的文献

1
A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosome.一种蛋白质在三个特定位点与卫星DNA重复序列结合，这些位点在核小体中会通过DNA折叠而相互靠近。
Cell. 1984 Jul;37(3):889-901. doi: 10.1016/0092-8674(84)90424-0.
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Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: location and function of the upstream activating sequence UASG.酿酒酵母GAL1 - GAL10双向启动子区域：上游激活序列UASG的定位与功能
Mol Cell Biol. 1984 Nov;4(11):2467-78. doi: 10.1128/mcb.4.11.2467-2478.1984.
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Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.
Sin3p的PAH结构域在酿酒酵母中提供了抑制减数分裂基因转录的不同功能。
Eukaryot Cell. 2010 Dec;9(12):1835-44. doi: 10.1128/EC.00143-10. Epub 2010 Oct 22.
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Meiosis-specific destruction of the Ume6p repressor by the Cdc20-directed APC/C.由Cdc20导向的后期促进复合物对Ume6p阻遏物进行减数分裂特异性破坏。
Mol Cell. 2007 Sep 21;27(6):951-61. doi: 10.1016/j.molcel.2007.08.019.
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Identification of silencer binding proteins from yeast: possible roles in SIR control and DNA replication.从酵母中鉴定沉默子结合蛋白：在SIR调控和DNA复制中的可能作用。
EMBO J. 1987 Feb;6(2):461-7. doi: 10.1002/j.1460-2075.1987.tb04776.x.
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A carbon-source-responsive element is required for regulation of the hypoxic ADP/ATP carrier (AAC3) isoform in Saccharomyces cerevisiae.在酿酒酵母中，缺氧ADP/ATP载体（AAC3）亚型的调控需要一个碳源响应元件。
Biochem J. 2000 Dec 15;352 Pt 3(Pt 3):893-8.
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Interactions of the yeast centromere and promoter factor, Cpf1p, with the cytochrome c1 upstream region and functional implications on regulated gene expression.酵母着丝粒与启动子因子Cpf1p与细胞色素c1上游区域的相互作用及其对基因表达调控的功能影响。
Nucleic Acids Res. 1996 Jun 15;24(12):2395-403. doi: 10.1093/nar/24.12.2395.
8
The CAGTCACA box in the fission yeast Schizosaccharomyces pombe functions like a TATA element and binds a novel factor.裂殖酵母粟酒裂殖酵母中的CAGTCACA框的功能类似于TATA元件，并结合一种新因子。
EMBO J. 1993 Mar;12(3):1201-8. doi: 10.1002/j.1460-2075.1993.tb05761.x.
9
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Sap1, a protein that binds to sequences required for mating-type switching, is essential for viability in Schizosaccharomyces pombe.Sap1是一种与交配型转换所需序列结合的蛋白质，对于粟酒裂殖酵母的生存能力至关重要。
Mol Cell Biol. 1994 Mar;14(3):2058-65. doi: 10.1128/mcb.14.3.2058-2065.1994.
利用lacZ融合来界定酿酒酵母中可诱导的双向GAL1 - GAL10启动子的调控元件。
Mol Cell Biol. 1984 Oct;4(10):1985-98. doi: 10.1128/mcb.4.10.1985-1998.1984.
4
Oxygen regulation of anaerobic and aerobic genes mediated by a common factor in yeast.酵母中由一个共同因子介导的厌氧与需氧基因的氧调节
Proc Natl Acad Sci U S A. 1984 Oct;81(19):6129-33. doi: 10.1073/pnas.81.19.6129.
5
Yeast RNA polymerase II initiates transcription in vitro at TATA sequences proximal to potential non-B forms of the DNA template.酵母RNA聚合酶II在体外于靠近DNA模板潜在非B型结构的TATA序列处起始转录。
EMBO J. 1984 May;3(5):1067-73. doi: 10.1002/j.1460-2075.1984.tb01929.x.
6
Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae.明显受调控的串联上游激活位点介导酿酒酵母CYC1基因的分解代谢物阻遏。
Cell. 1984 Feb;36(2):503-11. doi: 10.1016/0092-8674(84)90243-5.
7
Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site.血红素通过一个上游激活位点调节酿酒酵母CYC1基因的转录。
Cell. 1983 Apr;32(4):1279-86. doi: 10.1016/0092-8674(83)90309-4.
8
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳研究乳糖阻遏物-操纵基因相互作用的平衡与动力学
Nucleic Acids Res. 1981 Dec 11;9(23):6505-25. doi: 10.1093/nar/9.23.6505.
9
A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.一种用于定量蛋白质与特定DNA区域结合的凝胶电泳方法：应用于大肠杆菌乳糖操纵子调控系统的组分
Nucleic Acids Res. 1981 Jul 10;9(13):3047-60. doi: 10.1093/nar/9.13.3047.
10
Deletion mapping of sequences essential for in vivo transcription of the iso-1-cytochrome c gene.异-1-细胞色素c基因体内转录所需序列的缺失图谱分析。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2258-62. doi: 10.1073/pnas.78.4.2258.