Witt I, Straub N, Käufer N F, Gross T
Institut für Biochemie und Molekularbiologie, Freie Universität Berlin, Germany.
EMBO J. 1993 Mar;12(3):1201-8. doi: 10.1002/j.1460-2075.1993.tb05761.x.
Fourteen ribosomal protein genes from the fission yeast Schizosaccharomyces pombe contain a highly conserved sequence, CAGTCACA, in the proximal promoter. This sequence, which was also conserved in its location, was found where the TATA element usually resides. Deletion and point mutations in the CAGTCACA box reduced the expression of these genes to almost zero and caused aberrant transcriptional start sites. Insertions between this box and the original transcriptional start sites led to new start sites which were the same distance from the CAGTCACA box as the original start sites. The results presented provide evidence that this box, like a TATA sequence, is involved in basal expression and fixing the transcriptional start sites of these genes. Furthermore, the CAGTCACA sequence is the target of a binding protein which appears to be different from the TATA-binding protein.
来自裂殖酵母粟酒裂殖酵母的14个核糖体蛋白基因在近端启动子中含有高度保守的序列CAGTCACA。该序列在其位置上也保守，位于通常TATA元件所在之处。CAGTCACA框中的缺失和点突变将这些基因的表达降低到几乎为零，并导致异常的转录起始位点。在该框与原始转录起始位点之间的插入导致了新的起始位点，这些新起始位点与CAGTCACA框的距离与原始起始位点相同。所呈现的结果提供了证据，表明这个框像TATA序列一样，参与基础表达并确定这些基因的转录起始位点。此外，CAGTCACA序列是一种结合蛋白的靶标，该结合蛋白似乎不同于TATA结合蛋白。
