Aljindan Reem Y, Hussein Nasreldin E, Khoudair Hala A, Shaikh Alaa Y, Hassan Hoda A, Alabdulqader Nawar A, Shorman Mahmoud A, Abdalhamid Baha A
Department of Microbiology, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Kingdom of Saudi Arabia. E-mail.
Saudi Med J. 2018 Jul;39(7):685-689. doi: 10.15537/smj.2018.7.22532.
To detect resistance genes to fluoroquinolones and β-lactams in Salmonella strains from a Saudi hospital.
From October 2015 to December 2016, a total of 149 Salmonella strains were collected from stool specimens from patients admitted to King Fahad Hospital of the University, AlKhobar, Saudi Arabia using CHROMagar Salmonella. The organism identification and antimicrobial susceptibility testing were performed using Vitek 2 system. Strain serogrouping was performed using Wellcolex color Salmonella kit. Fluoroquinolone resistance genes, extended-spectrum β-lactamases (ESBLs), and AmpC β-lactamase were determined using polymerase chain reaction (PCR). Enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) was used to determine clonal relatedness.
The resistance rates to cefotaxime were 1.3% and ciprofloxacin 19.5%. Plasmid mediated quinolone resistance (PMQR) genes, qnrB and qnrS, were detected in 8 strains, qnrB (n=5) and qnrS (n=3), respectively. No ESBLs, AmpC, or mutations in the topoisomerases were detected. Salmonella isolates formed 7 clusters with similarity.
This study reveals the emergence of fluoroquinolone resistant Salmonella in the region imposing public health concerns.
检测沙特一家医院沙门氏菌菌株中对氟喹诺酮类和β-内酰胺类的耐药基因。
2015年10月至2016年12月，使用CHROMagar沙门氏菌培养基从沙特阿拉伯胡拜尔法赫德国王大学医院收治患者的粪便标本中收集了149株沙门氏菌菌株。使用Vitek 2系统进行菌种鉴定和抗菌药物敏感性试验。使用Wellcolex彩色沙门氏菌试剂盒进行菌株血清分型。使用聚合酶链反应（PCR）检测氟喹诺酮耐药基因、超广谱β-内酰胺酶（ESBLs）和AmpCβ-内酰胺酶。基于肠杆菌重复基因间共有序列的PCR（ERIC-PCR）用于确定克隆相关性。
对头孢噻肟的耐药率为1.3%，对环丙沙星的耐药率为19.5%。在8株菌株中检测到质粒介导的喹诺酮耐药（PMQR）基因，分别为qnrB（n = 5）和qnrS（n = 3）。未检测到ESBLs、AmpC或拓扑异构酶的突变。沙门氏菌分离株形成了7个相似性簇。
本研究揭示了该地区出现的氟喹诺酮耐药沙门氏菌，引起了公共卫生关注。
