Zhang Shuai, Gilbert Elizabeth R, Saremi Behnam, Wong Eric A
Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, Virginia.
Evonik Nutrition & Care GmbH, Hanau, Germany.
J Anim Physiol Anim Nutr (Berl). 2018 Oct;102(5):1274-1283. doi: 10.1111/jpn.12946. Epub 2018 Jul 3.
To determine the effect of different dietary Met sources on oxidative status, male Cobb 500 broiler chickens were fed from day of hatch to 26 days of age (d26) a diet deficient in sulfur amino acids (control) or a diet containing 0.22% DL-Met, 0.22% L-Met or 0.31% Met precursor, DL-2-hydroxy-4-(methylthio) butanoic acid (DL-HMTBA) to meet the Met + Cys requirements. Liver, breast muscle, duodenum, jejunum, and ileum were collected at day 10 (d10) and d26 to assay markers of oxidative stress, including total glutathione (TGSH), oxidized glutathione (GSSG), reduced glutathione (rGSH), protein carbonyls, thiobarbituric acid reactive substances (TBARS) and ferric reducing/antioxidant power (FRAP). In breast muscle, TGSH and rGSH were greater in L-Met and DL-HMTBA groups than in the control group (p < 0.05). An interaction of treatment and age was observed for TGSH in ileum (p = 0.01) and jejunum (p = 0.01), for GSSG in jejunum (p < 0.001), and for rGSH in ileum (p = 0.02). The ratios of rGSH to GSSG and GSSG to TGSH, which define oxidative status, were not affected by Met source. Protein carbonyls varied among groups in jejunum (p = 0.05) and breast muscle (p < 0.001), but were in the normal physiological range. No difference among treatment groups was observed for TBARS and FRAP in different tissues. Age effects were observed in all tissues for multiple oxidative stress markers. In conclusion, consuming different sources of supplementary Met did not alter the oxidative status in several tissues of broilers. Met + Cys deficiency did not compromise antioxidant capacity of chickens although growth was retarded.
为确定不同日粮蛋氨酸来源对氧化状态的影响，从孵化日起至26日龄（d26），给雄性科宝500肉鸡饲喂缺乏含硫氨基酸的日粮（对照组）或含有0.22% DL-蛋氨酸、0.22% L-蛋氨酸或0.31%蛋氨酸前体DL-2-羟基-4-（甲硫基）丁酸（DL-HMTBA）的日粮，以满足蛋氨酸+半胱氨酸的需求。在第10天（d10）和d26采集肝脏、胸肌、十二指肠、空肠和回肠，检测氧化应激标志物，包括总谷胱甘肽（TGSH）、氧化型谷胱甘肽（GSSG）、还原型谷胱甘肽（rGSH）、蛋白质羰基、硫代巴比妥酸反应性物质（TBARS）和铁还原/抗氧化能力（FRAP）。在胸肌中，L-蛋氨酸和DL-HMTBA组的TGSH和rGSH高于对照组（p < 0.05）。在回肠（p = 0.01）和空肠（p = 0.01）中观察到处理与年龄对TGSH有交互作用，在空肠中对GSSG有交互作用（p < 0.001），在回肠中对rGSH有交互作用（p = 0.02）。定义氧化状态的rGSH与GSSG以及GSSG与TGSH的比值不受蛋氨酸来源的影响。蛋白质羰基在空肠（p = 0.05）和胸肌（p < 0.001）的各组间有所不同，但处于正常生理范围内。不同处理组在不同组织中的TBARS和FRAP未观察到差异。在所有组织中均观察到多种氧化应激标志物的年龄效应。总之，采食不同来源的补充蛋氨酸并未改变肉鸡多个组织的氧化状态。尽管生长受阻，但蛋氨酸+半胱氨酸缺乏并未损害鸡的抗氧化能力。
