Liu ShuDong, Song MinHo, Yun Won, Lee JiHwan, Lee ChangHee, Kwak WooGi, Han NamSoo, Kim HyeunBum, Cho JinHo
Division of Food and Animal Science, Chungbuk National University, Cheongju-si, Korea.
Department of Animal Science and Biotechnology, Chungnam National University, Daejeon, Korea.
J Anim Physiol Anim Nutr (Berl). 2018 Oct;102(5):1257-1265. doi: 10.1111/jpn.12944. Epub 2018 Jul 3.
Essential oils are widely used in the pharmaceutical, food and cosmetic industries, and many plant essential oils have shown that they have positive effects on broilers nutrition. This experiment was conducted to study the effects of orally administered different dosages of carvacrol essential oils on intestinal barrier function in broiler chickens. A total of eighty 28-day-old (1.28 ± 0.15 kg) ROSS 308 broilers were randomly allocated to four groups of 20 replicates each, with one chicken per replicate per cage, and all were fed with the same diet. Four experimental groups were orally administered 0, 200, 300 or 400 μl carvacrol essential oils at 18:00 hr every day during the 2-week experimental period. As a result of which, the gene expression of the occludin, claudin-1, claudin-5, ZO-1 and ZO-2 in intestinal mucosa of small intestine (p < 0.05) and the goblet cell content in small intestine epithelium (p < 0.05) were significantly increased; test subjects with 300 or 400 μl carvacrol essential oils reduced the microbial counts of Salmonella spp. and Escherichia coli in the intestines (p < 0.05); Essential oils administration also significantly increased activity of the sucrase (p < 0.05) and lactase (p < 0.05) in intestinal mucosa. In conclusion, the carvacrol essential oils have positive effects on growth performance and intestinal barriers function of broilers; those effects may be related to the dosage, as administration of 300 or 400 μl was more effective than that of 200 μl.
香精油广泛应用于制药、食品和化妆品行业，许多植物香精油已表明它们对肉鸡营养有积极作用。本试验旨在研究口服不同剂量香芹酚香精油对肉鸡肠道屏障功能的影响。总共80只28日龄（1.28±0.15千克）的ROSS 308肉鸡被随机分为四组，每组20个重复，每个笼子每个重复一只鸡，所有鸡均饲喂相同的日粮。在为期2周的试验期内，每天18:00给四个试验组分别口服0、200、300或400微升香芹酚香精油。结果显示，小肠肠黏膜中闭合蛋白、Claudin-1、Claudin-5、ZO-1和ZO-2的基因表达（p<0.05）以及小肠上皮杯状细胞含量（p<0.05）显著增加；口服300或400微升香芹酚香精油的试验对象肠道中沙门氏菌和大肠杆菌的微生物数量减少（p<0.05）；香精油给药还显著提高了肠黏膜中蔗糖酶（p<0.05）和乳糖酶（p<0.05）的活性。总之，香芹酚香精油对肉鸡的生长性能和肠道屏障功能有积极作用；这些作用可能与剂量有关，因为口服300或400微升比200微升更有效。
