Friedland D M, Ades I Z
FEBS Lett. 1985 Oct 21;191(1):117-20. doi: 10.1016/0014-5793(85)81005-x.
In primary cultures of chick embryo hepatocytes pulse labeled with [35S]methionine, immunochemical analyses indicated that adenosine 3':5'-cyclic monophosphate (cAMP) did not affect either the rate of production or the maturation of delta-aminolevulinate synthase (ALA synthase). In addition, allylisopropylacetamide caused a slight drop in intracellular cAMP while testosterone caused the levels of cAMP to rise to 260% of the basal levels measured in hepatocytes in culture. Thus the results of this study did not indicate a direct short-term role for cAMP in the regulation of production of ALA synthase.
在用[35S]甲硫氨酸脉冲标记的鸡胚肝细胞原代培养物中，免疫化学分析表明，3':5'-环磷酸腺苷（cAMP）既不影响δ-氨基乙酰丙酸合酶（ALA合酶）的产生速率，也不影响其成熟过程。此外，烯丙基异丙基乙酰胺使细胞内cAMP略有下降，而睾酮则使cAMP水平升至培养肝细胞中测得的基础水平的260%。因此，本研究结果并未表明cAMP在ALA合酶产生的调节中具有直接的短期作用。
