Tsuda T, Kaibuchi K, Kawahara Y, Fukuzaki H, Takai Y
FEBS Lett. 1985 Oct 28;191(2):205-10. doi: 10.1016/0014-5793(85)80009-0.
Addition of fibroblast growth factor (FGF) to quiescent cultures of Swiss 3T3 cells rapidly induced diacylglycerol formation, protein kinase C activation and Ca2+ mobilization. Protein kinase C-activating agents such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and 1-oleoyl-2-acetylglycerol (OAG) mimicked the action of FGF and stimulated DNA synthesis in the presence of insulin. Prolonged treatment of the cells with phorbol-12,13-dibutyrate (PDBu) led to the down-regulation and complete disappearance of protein kinase C. In these cells, TPA and OAG did not induce DNA synthesis any more. FGF still elicited Ca2+ mobilization and DNA synthesis, but the magnitude of DNA synthesis was reduced to almost half as compared with that in the control cells. These results clearly indicate that both diacylglycerol and Ca2+ may serve as second messengers for FGF and suggest that these messengers may be involved in the mitogenic action of this growth factor.
向静止的瑞士3T3细胞培养物中添加成纤维细胞生长因子（FGF）可迅速诱导二酰基甘油的形成、蛋白激酶C的激活以及钙离子动员。蛋白激酶C激活剂，如12 - O - 十四酰佛波醇 - 13 - 乙酸酯（TPA）和1 - 油酰 - 2 - 乙酰甘油（OAG），模拟了FGF的作用，并在胰岛素存在的情况下刺激DNA合成。用佛波醇 - 12,13 - 二丁酸酯（PDBu）对细胞进行长时间处理导致蛋白激酶C的下调和完全消失。在这些细胞中，TPA和OAG不再诱导DNA合成。FGF仍能引发钙离子动员和DNA合成，但与对照细胞相比，DNA合成的幅度降低到几乎一半。这些结果清楚地表明，二酰基甘油和钙离子都可能作为FGF的第二信使，并表明这些信使可能参与了这种生长因子的促有丝分裂作用。
