Kaibuchi K, Miyajima A, Arai K, Matsumoto K
Proc Natl Acad Sci U S A. 1986 Nov;83(21):8172-6. doi: 10.1073/pnas.83.21.8172.
Incubation of yeast Saccharomyces cerevisiae at very low (0.02%) glucose levels led to arrest of the cell cycle at the G0/G1 phase. Readdition of glucose to these "starved" yeast resulted in cell proliferation. In glucose-starved yeast, glucose stimulated 32P incorporation into phosphatidic acid, phosphatidylinositol, phosphatidylinositol monophosphate, and phosphatidylinositol bisphosphate but not into phosphatidylethanolamine and phosphatidylcholine. Preincubation of yeast with [3H]inositol and subsequent exposure to glucose resulted in rapid formation of [3H]inositol monophosphate and [3H]inositol trisphosphate, presumably derived from phosphatidylinositol and phosphatidylinositol bisphosphate. Under similar conditions, glucose elicited both efflux and influx of Ca2+ in yeast. Glucose-induced 32P incorporation into inositolphospholipids and formation of [3H]inositol phosphates were more pronounced in RAS-related mutants such as ras1, ras1 ras2 bcy1, and RAS2Val19 than in the wild-type strain. These results strongly suggest that glucose stimulates inositolphospholipid turnover, Ca2+ mobilization, and subsequent cell proliferation in a manner similar to that of growth factors with mammalian cells, and that RAS-encoded proteins are involved in regulation of this glucose-induced inositolphospholipid turnover in yeast.
将酿酒酵母在极低（0.02%）葡萄糖水平下培养会导致细胞周期在G0/G1期停滞。向这些“饥饿”酵母中重新添加葡萄糖会导致细胞增殖。在葡萄糖饥饿的酵母中，葡萄糖会刺激32P掺入磷脂酸、磷脂酰肌醇、磷脂酰肌醇单磷酸和磷脂酰肌醇二磷酸，但不会掺入磷脂酰乙醇胺和磷脂酰胆碱。用[3H]肌醇预孵育酵母，随后暴露于葡萄糖会导致[3H]肌醇单磷酸和[3H]肌醇三磷酸迅速形成，推测它们来源于磷脂酰肌醇和磷脂酰肌醇二磷酸。在类似条件下，葡萄糖会引起酵母中Ca2+的外流和内流。葡萄糖诱导的32P掺入肌醇磷脂以及[3H]肌醇磷酸的形成在RAS相关突变体（如ras1、ras1 ras2 bcy1和RAS2Val19）中比在野生型菌株中更明显。这些结果强烈表明，葡萄糖以类似于哺乳动物细胞生长因子的方式刺激肌醇磷脂周转、Ca2+动员以及随后的细胞增殖，并且RAS编码的蛋白质参与酵母中这种葡萄糖诱导的肌醇磷脂周转的调节。
