Yavas Cagdas, Yavas Guler, Celik Esin, Buyukyoruk Ahmet, Buyukyoruk Cennet, Yuce Deniz, Ata Ozlem
Selcuk University, Department of Radiation Oncology , Konya , Turkey.
Selcuk University, Department of Pathology , Konya , Turkey.
J Diet Suppl. 2019;16(5):576-591. doi: 10.1080/19390211.2018.1472709. Epub 2018 Jul 3.
We aimed to evaluate effects of β-hydroxy-β-methylbutyrate, L-glutamine, and L-arginine (HMB/GLN/ARG) on radiation-induced acute intestinal toxicity. Forty rats were divided into four groups: group (G) 1 was defined as control group, and G2 was radiation therapy (RT) control group. G3 and G4 were HMB/GLN/ARG control and RT plus HMB/GLN/ARG groups, respectively. HMB/GLN/ARG started from day of RT and continued until the animals were sacrificed 10 days after RT. The extent of surface epithelium smoothing, villous atrophy, lamina propria inflammation, cryptitis, crypt distortion, regenerative atypia, vascular dilatation and congestion, and fibrosis were quantified on histological sections of intestinal mucosa. Statistical analyses were performed using the analysis of variance (ANOVA) test. There were significant differences between study groups regarding extent of surface epithelium smoothing, villous atrophy, lamina propria inflammation, cryptitis and crypt distortion, regenerative atypia, vascular dilatation and congestion, and fibrosis ( values were 0.019 for fibrosis, <.001 for the others). Pair-wise comparisons revealed significant differences regarding surface epithelium smoothing, villous atrophy, lamina propria inflammation, cryptitis, vascular dilatation, and congestion between G2 and G4 ( values were <.001, .033, <.001, .007, and <.001, respectively). Fibrosis score was significantly different only between G1 and G2 ( = .015). Immunohistochemical TGF-β score of G2 was significantly higher than G1 and G3 ( values were .006 and .017, respectively). There was no difference between TGF-β staining scores of G2 and G4. Concomitant use of HMB/GLN/ARG appears to ameliorate radiation-induced acute intestinal toxicity; however, this finding should be clarified with further studies.
我们旨在评估β-羟基-β-甲基丁酸、L-谷氨酰胺和L-精氨酸（HMB/GLN/ARG）对辐射诱导的急性肠道毒性的影响。40只大鼠被分为四组：第1组（G1）定义为对照组，第2组（G2）为放射治疗（RT）对照组。第3组（G3）和第4组（G4）分别为HMB/GLN/ARG对照组和RT加HMB/GLN/ARG组。HMB/GLN/ARG从放疗当天开始使用，持续至放疗后10天处死动物。对肠黏膜组织切片上表面上皮平滑度、绒毛萎缩、固有层炎症、隐窝炎、隐窝变形、再生异型性、血管扩张和充血以及纤维化的程度进行量化。使用方差分析（ANOVA）检验进行统计分析。研究组之间在表面上皮平滑度、绒毛萎缩、固有层炎症、隐窝炎和隐窝变形、再生异型性、血管扩张和充血以及纤维化程度方面存在显著差异（纤维化的P值为0.019，其他的P值均<0.001）。两两比较显示，G2和G4之间在表面上皮平滑度、绒毛萎缩、固有层炎症、隐窝炎、血管扩张和充血方面存在显著差异（P值分别为<0.001、0.033、<0.001、0.007和<0.001）。纤维化评分仅在G1和G2之间存在显著差异（P = 0.015）。G2的免疫组化TGF-β评分显著高于G1和G3（P值分别为0.006和0.017）。G2和G4的TGF-β染色评分之间没有差异。同时使用HMB/GLN/ARG似乎可改善辐射诱导的急性肠道毒性；然而，这一发现应通过进一步研究加以阐明。
