Department of General Surgery, University of Health Sciences, Şişli Hamidiye Etfal Training and Research Hospital, İstanbul, Turkiye.
Department of Pathology, University of Health Sciences, Şişli Hamidiye Etfal Training and Research Hospital, İstanbul, Turkiye.
Turk J Med Sci. 2024 Jan 5;54(1):59-68. doi: 10.55730/1300-0144.5766. eCollection 2024.
BACKGROUND/AIM: Intestinal neomucosa formation is a technique defined for the treatment of short bowel syndrome. This study evaluates the effect of glutamine and omega-3 fatty acids on the growth of intestinal neomucosa on the colonic serosal surface has been evaluated.
Thirty-two adult male Sprague-Dawley rats were randomly divided into 4 groups: sham, control, glutamine, and omega-3. Laparotomy was performed on all groups. For rats other than the sham group, a 1-cm full-thickness incision was made 4 cm proximal to the ileocecal valve, and colonic serosal surface was sutured as a serosal patch over these openings. By using the oral gavage technique, the glutamine group was ingested with 200 mg/kg/day of glutamine, and the omega-3 group was ingested with 100 mg/kg/day of omega-3 fatty acids. At the end of 14 days, the rats were euthanized, blood specimens were collected, and intestinal segments, including serosal patches, were excised.
Transforming growth factor-beta was significantly lower in the glutamine group compared to the control group. Similarly, fibroblast growth factor-2 was significantly lower in the glutamine group compared to the sham group. Intestinal neomucosa formation was observed in 100% of rats in the glutamine group. In the control and omega-3 groups, intestinal neomucosa formation was observed in 57.1% and 60% of rats, respectively. The inflammatory response, granulation tissue formation, and fibroblastic activity were more severe in the rats of the glutamine and omega-3 groups.
The intestinal neomucosa formation is an experimental technique, and both glutamine and omega-3 fatty acids have the potential to positively affect inflammatory response, granulation tissue formation, and fibroblastic activity. Specifically, glutamine has a favorable effect on intestinal neomucosa formation.
背景/目的：肠黏膜新生是一种用于治疗短肠综合征的技术。本研究评估了谷氨酰胺和ω-3 脂肪酸对结肠浆膜表面肠黏膜新生的生长的影响。
32 只成年雄性 Sprague-Dawley 大鼠随机分为 4 组：假手术组、对照组、谷氨酰胺组和 ω-3 组。所有组均行剖腹术。除假手术组外，其余各组大鼠在回盲瓣近端 4cm 处做 1cm 全层切口，将结肠浆膜表面缝合为浆膜补丁覆盖这些开口。通过口服灌胃技术，谷氨酰胺组每天摄入 200mg/kg 的谷氨酰胺，ω-3 组每天摄入 100mg/kg 的 ω-3 脂肪酸。14 天后处死大鼠，采集血标本，并切除包括浆膜补丁在内的肠段。
与对照组相比，谷氨酰胺组转化生长因子-β显著降低。同样，与假手术组相比，谷氨酰胺组成纤维细胞生长因子-2 显著降低。谷氨酰胺组 100%的大鼠观察到肠黏膜新生。在对照组和 ω-3 组中，分别有 57.1%和 60%的大鼠观察到肠黏膜新生。谷氨酰胺组和 ω-3 组的大鼠炎症反应、肉芽组织形成和纤维母细胞活性更为严重。
肠黏膜新生是一种实验技术，谷氨酰胺和 ω-3 脂肪酸都有可能对炎症反应、肉芽组织形成和纤维母细胞活性产生积极影响。具体来说，谷氨酰胺对肠黏膜新生有有利影响。
