中国西藏地区双孢蘑菇多糖对四氯化碳诱导的小鼠急性肝损伤的保护作用。

Protective effect of polysaccharide from Agaricus bisporus in Tibet area of China against tetrachloride-induced acute liver injury in mice.

机构信息

Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130012, China.

出版信息

Int J Biol Macromol. 2018 Oct 15;118(Pt B):1488-1493. doi: 10.1016/j.ijbiomac.2018.06.179. Epub 2018 Jun 30.

DOI:10.1016/j.ijbiomac.2018.06.179

PMID:29969638

Abstract

In this study, we systematically investigated the physicochemical properties, hepatoprotective activities and the underlying mechanisms of Agaricus bisporus polysaccharide (FPS). The physicochemical evaluations indicate that FPS is mainly composed of glucose, mannose, galactose, fucose, arabinose and glucuronic acid with the mole percentages of 70.30%, 8.70%, 12.88%, 0.79%, 5.04% and 1.57%, respectively. The results of methylation analysis indicated that FPS mainly included 1-linked-glc, 1,4-linked-glc, 1,6-linked-glc, 1,3-linked-gal and 1,3,6-linked-man glycosidic bonds. The results from hepatoprotective studies showed that FPS significantly decreased the liver index, serum gamma-glutamyltranspeptidase (GGT), alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST) activities and malondialdehyde (MDA) contents in liver tissue, and increased antioxidant capacities of hepatic glutathione (GSH) and superoxide dismutase (SOD). Furthermore, histological examination of liver tissues showed that FPS alleviated CCl-induced liver injury. Finally, the Western blot results showed that FPS could significantly decrease the expression levels of TGF-β1 and Smad3 in the liver. These results together suggest that FPS possesses hepatoprotective effect against CCl-induced liver damage, in part through the downregulation of TGF-β1/Smad signaling pathway, therefore FPS could be considered as a hepatoprotective medicine or functional food.

摘要

在这项研究中，我们系统地研究了双孢蘑菇多糖（FPS）的理化性质、保肝活性及其作用机制。理化评价表明，FPS 主要由葡萄糖、甘露糖、半乳糖、岩藻糖、阿拉伯糖和葡萄糖醛酸组成，摩尔百分比分别为 70.30%、8.70%、12.88%、0.79%、5.04%和 1.57%。甲基化分析结果表明，FPS 主要含有 1 位连接的葡萄糖、1,4 位连接的葡萄糖、1,6 位连接的葡萄糖、1,3 位连接的半乳糖和 1,3,6 位连接的甘露糖苷键。保肝研究结果表明，FPS 能显著降低肝指数、血清γ-谷氨酰转肽酶（GGT）、丙氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）、天门冬氨酸氨基转移酶（AST）活性和肝组织丙二醛（MDA）含量，提高肝组织谷胱甘肽（GSH）和超氧化物歧化酶（SOD）的抗氧化能力。此外，肝组织病理学检查表明，FPS 能减轻 CCl 诱导的肝损伤。最后，Western blot 结果表明，FPS 能显著降低肝组织中 TGF-β1 和 Smad3 的表达水平。这些结果表明，FPS 对 CCl 诱导的肝损伤具有保护作用，部分通过下调 TGF-β1/Smad 信号通路，因此 FPS 可作为一种保肝药物或功能性食品。