Department of Clinical Laboratory, Heilongjiang Provincial Hospital, Harbin, Heilongjiang Province 150036, China.
Department of Surgery, Heilongjiang Provincial Hospital, Harbin, Heilongjiang Province 150036, China.
Gene. 2018 Oct 30;675:88-93. doi: 10.1016/j.gene.2018.06.099. Epub 2018 Jun 30.
Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease with increasing incidence worldwide. Accumulating evidence indicated that circular RNAs (circRNAs) behave as a novel class of transcription products during multiple cancer processes. Specifically, hsa_circ_0001649 has been reported to be down-regulated in several cancers. However, its clinical significance and functional roles in PDAC is still unknown. RT-qPCR was carried out to measure the expression of hsa_circ_0001649 in PDAC tissue samples and cell lines. Additionally, the correlation between hsa_circ_0001649 expression and clinicopathological features was analyzed. The prognostic role of hsa_circ_0001649 was explored by Cox regression analysis. The potential effects of hsa_circ_0001649 in PDAC cells were evaluated in vitro including cell proliferation, colony-forming ability and apoptosis. As a result, hsa_circ_0001649 was abnormally decreased in PDAC tissues and cells, and this down-regulation was correlated with tumor stage and differentiation grade in PDAC patients. Hsa_circ_0001649 could serve as an independent prognostic factor for PDAC patients after surgery. What's more, increased hsa_circ_0001649 caused tumor suppressive effects via reducing cell proliferation, colony-forming ability and promoting cell apoptosis in PANC1 and BxPC3 cells. Collectively, the results illustrated that hsa_circ_0001649 may play a tumor suppressor role in PDAC and offer a potential therapeutic target for treating this fatal disease.
胰腺导管腺癌（PDAC）是一种具有全球发病率不断增加的毁灭性疾病。越来越多的证据表明，环状 RNA（circRNA）在多种癌症过程中表现为一类新型的转录产物。具体来说，已经有报道称 hsa_circ_0001649 在几种癌症中下调。然而，其在 PDAC 中的临床意义和功能作用尚不清楚。通过 RT-qPCR 测量 PDAC 组织样本和细胞系中 hsa_circ_0001649 的表达。此外，分析了 hsa_circ_0001649 表达与临床病理特征之间的相关性。通过 Cox 回归分析探讨了 hsa_circ_0001649 在 PDAC 中的预后作用。通过体外细胞增殖、集落形成能力和细胞凋亡实验评估了 hsa_circ_0001649 在 PDAC 细胞中的潜在作用。结果表明，hsa_circ_0001649 在 PDAC 组织和细胞中异常下调，这种下调与 PDAC 患者的肿瘤分期和分化程度相关。hsa_circ_0001649 可作为 PDAC 患者手术后的独立预后因素。此外，增加 hsa_circ_0001649 通过降低细胞增殖、集落形成能力和促进 PANC1 和 BxPC3 细胞凋亡来发挥肿瘤抑制作用。总之，这些结果表明 hsa_circ_0001649 可能在 PDAC 中发挥肿瘤抑制作用，并为治疗这种致命疾病提供了潜在的治疗靶点。
