编码对氨苄青霉素抗性增强的RP4质粒突变体的分离与鉴定。

Isolation and characterization of mutants of the RP4 plasmid coding for increased resistance to ampicillin.

作者信息

Cejka K, Holubová I, Hubácek J, Navrátil O, Braná H

出版信息

Folia Microbiol (Praha). 1985;30(5):401-6. doi: 10.1007/BF02928748.

Abstract

E. coli strain J53(RP4) was mutagenized with ethyl methanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine. Clones showing a two-to threefold increase in resistance to ampicillin were produced. This increase was not due to an increased number of RP4 copies per chromosome. The level of penicillinase activity was twice higher in comparison with the parental strain. No detectable changes were found in the region coding for the resistance to ampicillin on the plasmid by restriction analysis.

摘要

大肠杆菌菌株J53（RP4）用甲磺酸乙酯和N-甲基-N'-硝基-N-亚硝基胍进行诱变处理。产生了对氨苄青霉素抗性增加两到三倍的克隆。这种增加并非由于每条染色体上RP4拷贝数的增加。与亲本菌株相比，青霉素酶活性水平高出两倍。通过限制性分析，在质粒上编码氨苄青霉素抗性的区域未发现可检测到的变化。