Hochmannová J, Nesvera J
Folia Microbiol (Praha). 1982;27(2):138-41. doi: 10.1007/BF02879773.
An economic method for a rapid estimation of the number of copies of plasmid R6K delta 1 in E. coli cells using lysis of the cells directly on the agarose gel and electrophoretic separation of radioactively labelled plasmid and chromosomal DNA's is described. The method, particularly useful for screening purposes, permits detection of both the CCC and OC forms of plasmids of molar mass 2-150 Mg/mol and it can be applied to other bacterial systems.
描述了一种经济的方法，通过直接在琼脂糖凝胶上裂解细胞，并对放射性标记的质粒和染色体DNA进行电泳分离，快速估算大肠杆菌细胞中质粒R6Kδ1的拷贝数。该方法对于筛选目的特别有用，可检测摩尔质量为2 - 150 Mg/mol的质粒的CCC和OC形式，并且可应用于其他细菌系统。
