质粒不相容性与复制控制：大肠杆菌K-12中R因子R1的复制突变体
Plasmid incompatibility and control of replication: copy mutants of the R-factor R1 in Escherichia coli K-12.
作者信息
Uhlin B E, Nordström K
出版信息
J Bacteriol. 1975 Nov;124(2):641-9. doi: 10.1128/jb.124.2.641-649.1975.
Abstract
Plasmid incompatibility was studied in Escherichia coli K-12. By double-antibiotic selection, clones were constructed that carried the two R-factors R1 and R100, both belonging to the compatibility group FII. After release of the selection pressure, each of the two plasmids was lost at the same rate (8% per generation). Mutants of R-factor R1 showing an increased number of copies per chromosome (copy mutants) were tested for their incompatibility towards R-factor R100. The results indicate that plasmid incompatibility is quantitative and not just a qualitative property. All copy mutants studied affected incompatibility, and there were two classes of mutants: one increasing and one decreasing the incompatiblity exerted towards the test plasmid R100. Evidence is presented that incompatibility is related to the mechanisms that control replication. The implications of such a relation on proposed models for control of replication are discussed. The data do not support the hypothesis that plasmid incompatibility is due to competition for a replicational or segregational site.
摘要
在大肠杆菌K-12中研究了质粒不相容性。通过双抗生素选择，构建了携带两个R因子R1和R100的克隆，这两个R因子都属于相容性群FII。解除选择压力后，两种质粒以相同的速率（每代8%）丢失。对R因子R1的每个染色体拷贝数增加的突变体（拷贝突变体）进行了对R因子R100的不相容性测试。结果表明，质粒不相容性是定量的，而不仅仅是定性的特性。所研究的所有拷贝突变体都影响不相容性，并且有两类突变体：一类增加对测试质粒R100施加的不相容性，另一类降低不相容性。有证据表明不相容性与控制复制的机制有关。讨论了这种关系对提出的复制控制模型的影响。数据不支持质粒不相容性是由于对复制或分离位点的竞争这一假设。
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本文引用的文献
1
Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli.溶源性研究。I. 溶源性大肠杆菌释放噬菌体的方式。
J Bacteriol. 1951 Sep;62(3):293-300. doi: 10.1128/jb.62.3.293-300.1951.
2
GROWTH CHARACTERISITICS OF SOME GRAM-NEGATIVE BACTERIA.一些革兰氏阴性菌的生长特性
J Gen Microbiol. 1965 Apr;39:109-23. doi: 10.1099/00221287-39-1-109.
3
Generation times of individual bacteria: some corroborative measurements.单个细菌的世代时间：一些确证性测量
J Gen Microbiol. 1963 May;31:315-27. doi: 10.1099/00221287-31-2-315.
4
Growth, cell and nuclear divisions in some bacteria.某些细菌中的生长、细胞分裂和核分裂
J Gen Microbiol. 1962 Nov;29:421-34. doi: 10.1099/00221287-29-3-421.
5
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
J Biol Chem. 1956 Jan;218(1):97-106.
6
Some observations on linkage effects in genetic recombination in Escherichia coli K-12.关于大肠杆菌K-12遗传重组中连锁效应的一些观察
J Gen Microbiol. 1954 Oct;11(2):250-60. doi: 10.1099/00221287-11-2-250.
7
Mutant drug resistant factors of high transmissibility.具有高传播性的突变耐药因子。
Nature. 1967 May 27;214(5091):885-7. doi: 10.1038/214885a0.
8
The relation of resistance transfer factors to the F-factor (sex-factor) of Escherichia coli K12.大肠杆菌K12的抗性转移因子与F因子（性因子）的关系。
Genet Res. 1966 Feb;7(1):134-40. doi: 10.1017/s0016672300009538.
9
Colicin tolerance induced by ampicillin or mutation to ampicillin resistance in a strain of Escherichia coli K-12.氨苄青霉素诱导的大肠杆菌K - 12菌株对大肠杆菌素的耐受性或对氨苄青霉素抗性的突变。
J Bacteriol. 1971 Apr;106(1):1-13. doi: 10.1128/jb.106.1.1-13.1971.
10
The problems of drug-resistant pathogenic bacteria. The replication of R-factor DNA in Escherichia coli K-12 following conjugation.耐药病原菌的问题。接合后R因子DNA在大肠杆菌K-12中的复制。
Ann N Y Acad Sci. 1971 Jun 11;182:153-71. doi: 10.1111/j.1749-6632.1971.tb30654.x.
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