源自慢性粒细胞白血病细胞的外泌体作为生物标志物的潜力
The Potential of Exosomes Derived from Chronic Myelogenous Leukaemia Cells as a Biomarker.
作者信息
Kang Ka-Won, Jung Jik-Han, Hur Woojune, Park Jaena, Shin Hyunku, Choi Byeonghyeon, Jeong Hyesun, Kim Dae Sik, Yu Eun Sang, Lee Se Ryeon, Sung Hwa Jung, Kim Seok Jin, Choi Chul Won, Kim Hyun Koo, Hong Sunghoi, Park Ji-Ho, Choi Yeonho, Park Yong, Kim Byung Soo
机构信息
Division of Hematology-Oncology, Department of Internal Medicine, Korea University College of Medicine, Seoul, Republic of Korea.
Department of Bio and Brain Bioengineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
出版信息
Anticancer Res. 2018 Jul;38(7):3935-3942. doi: 10.21873/anticanres.12679.
BACKGROUND/AIM: Exosomes, derived from chronic myelogenous leukaemia (CML) cells, can be used as biomarkers and new targets for the detection of the BCR-ABL transcript. This study aimed to identify these possibilities.
MATERIALS AND METHODS
Human CML cell line-derived exosomes and CML-patients-derived exosomes were isolated with a size-exclusion chromatography column and ExoQuick™ exosome precipitation solution, respectively. Isolated exosomes were analysed by nested PCR to detect the BCR-ABL transcript.
RESULTS
Exosomes derived from the two human CML cell lines yielded a 250-bp band. RNA sequence analysis revealed 99% sequence homology with the partial mRNA for the human BCR-ABL chimeric protein. This ~250-bp band was also observed in the exosomes derived from patients with CML. However, only patients at the blast and accelerated phases showed the exosomal BCR-ABL transcript.
CONCLUSION
CML-derived exosomes could act as novel targets for the detection of the BCR-ABL transcript.
背景/目的：源自慢性粒细胞白血病（CML）细胞的外泌体可作为检测BCR-ABL转录本的生物标志物和新靶点。本研究旨在确定这些可能性。
材料与方法
分别使用尺寸排阻色谱柱和ExoQuick™外泌体沉淀溶液分离人CML细胞系来源的外泌体和CML患者来源的外泌体。通过巢式PCR分析分离的外泌体以检测BCR-ABL转录本。
结果
源自两个人CML细胞系的外泌体产生了一条250bp的条带。RNA序列分析显示与人类BCR-ABL嵌合蛋白的部分mRNA有99%的序列同源性。在CML患者来源的外泌体中也观察到了这条~250bp的条带。然而，只有处于原始细胞期和加速期的患者显示出外泌体BCR-ABL转录本。
结论
CML来源的外泌体可作为检测BCR-ABL转录本的新靶点。
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