Arimoto Akira, Yamashita Kimihiro, Hasegawa Hiroshi, Sugita Yutaka, Fukuoka Eiji, Tanaka Tomoko, Suzuki Satoshi, Kakeji Yoshihiro
Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan
Anticancer Res. 2018 Jul;38(7):4333-4338. doi: 10.21873/anticanres.12733.
BACKGROUND/AIM: Perioperative intra-abdominal infection has been reported as a risk factor for metastasis. The aim of this study was to investigate the mechanism by which peritonitis induces immunosuppression in the lung, which in turn promotes lung metastasis.
C57BL/6 mice were intravenously administered B16F10 melanoma cells to induce lung metastasis and subsequently subjected to cecal ligation and puncture (CLP) to induce peritonitis or sham surgery. The number of lung metastatic nodules was evaluated. Cell fractions in lungs and serum cytokines after CLP were investigated.
CLP mice showed an increased number of lung metastases compared to sham-treated mice. The fraction and number of natural killer (NK) cells in lungs of CLP mice were significantly reduced in early post-CLP phase. Myeloid-derived suppressor cells (MDSCs) in lungs were significantly decreased in CLP mice. Serum IL-6 and TNF levels were significantly elevated in CLP mice.
Peritonitis promoted lung metastasis in a murine model, which may be attributable to the impact of NK cells and MDSCs in the lungs.
背景/目的：围手术期腹腔内感染已被报道为转移的危险因素。本研究的目的是探讨腹膜炎诱导肺免疫抑制进而促进肺转移的机制。
给C57BL/6小鼠静脉注射B16F10黑色素瘤细胞以诱导肺转移，随后进行盲肠结扎和穿刺（CLP）以诱导腹膜炎或假手术。评估肺转移结节的数量。研究CLP后肺中的细胞组分和血清细胞因子。
与假手术组小鼠相比，CLP小鼠的肺转移数量增加。CLP小鼠肺中自然杀伤（NK）细胞比例和数量在CLP后早期显著降低。CLP小鼠肺中的髓源性抑制细胞（MDSC）显著减少。CLP小鼠血清IL-6和TNF水平显著升高。
腹膜炎在小鼠模型中促进了肺转移，这可能归因于肺中NK细胞和MDSC的影响。
