Yamashiro D, Li C H
Int J Pept Protein Res. 1985 Sep;26(3):299-304. doi: 10.1111/j.1399-3011.1985.tb03208.x.
Human insulin-like growth factor II (IGF-II) with 67 amino acids and three disulfide bridges has been synthesized by the solid-phase method. The synthetic hormone is shown to be homogeneous in high performance liquid chromatography (HPLC), high performance partition chromatography (HPPC), and chromatofocusing. It is indistinguishable from natural hormone in HPLC, peptide map of thermolysin digests, amino acid composition and radioreceptor binding assay. Thus, synthetic and natural IGF-II are identical.
含67个氨基酸和三个二硫键的人胰岛素样生长因子II（IGF-II）已通过固相法合成。该合成激素在高效液相色谱（HPLC）、高效分配色谱（HPPC）和色谱聚焦法中显示为均一的。在HPLC、嗜热菌蛋白酶消化产物的肽图、氨基酸组成和放射受体结合试验中，它与天然激素没有区别。因此，合成的IGF-II与天然的IGF-II是相同的。
