Li C H, Yamashiro D, Hammonds R G, Westphal M
Biochem Biophys Res Commun. 1985 Mar 15;127(2):420-4. doi: 10.1016/s0006-291x(85)80177-7.
Human insulin-like growth factor II with 67 amino acid residues and three disulfide bridges has been synthesized by the solid-phase method. Homogeneity of the synthetic product is ascertained by chromatofocusing, high performance liquid chromatography and amino acid analysis. In both radioimmunoassay and radioreceptor assay, the synthetic product is indistinguishable from the natural hormone.