Wojtecka-Lukasik E, Maśliński S
Int J Tissue React. 1985;7(5):387-90.
The intrapleural injection of carrageenin into rats caused accumulation of leucocytes in the pleural cavity and changes in blood leucocyte populations. An increased number of polymorphonuclear (PMN) leucocytes was observed. The peripheral blood PMN leucocytes of untreated rats contained 33.2 nM reduced glutathione/10(7) cells and collagenase mainly in the latent form. During experimental pleurisy in blood leucocytes as well as in the leucocytes obtained from the pleural exudate, the level of reduced glutathione decreased significantly by about 33% (22.4 nM/10(7) cells) and parallel collagenase activation up to 80-100% was observed. Two tested antiinflammatory drugs, Timegadine and piroxicam, which significantly inhibited activation of latent collagenase during the course of inflammation and partially protected reduced glutathione against oxidation. These results suggest that the activation of latent leucocyte collagenase in vivo via the disulphide-thiol interchange reaction may be coupled to the glutathione cycle and regulated by variation in its redox potential.
向大鼠胸腔内注射角叉菜胶会导致胸腔内白细胞积聚以及血液白细胞群体发生变化。观察到多形核（PMN）白细胞数量增加。未处理大鼠的外周血PMN白细胞含有33.2 nM还原型谷胱甘肽/10⁷细胞，且胶原酶主要以潜伏形式存在。在实验性胸膜炎期间，血液白细胞以及从胸腔渗出液中获得的白细胞中，还原型谷胱甘肽水平显著降低约33%（22.4 nM/10⁷细胞），同时观察到平行的胶原酶激活率高达80 - 100%。两种受试抗炎药物，替马加定和吡罗昔康，在炎症过程中显著抑制潜伏胶原酶的激活，并部分保护还原型谷胱甘肽免受氧化。这些结果表明，体内潜伏白细胞胶原酶通过二硫键 - 硫醇交换反应的激活可能与谷胱甘肽循环相关，并受其氧化还原电位变化的调节。
