CHU Nantes, Pharmacie Centrale, PHU 11, F-44093 Nantes, France.
INRA UR1268, Biopolymères Interactions Assemblages, F-44300 Nantes, France.
Int J Mol Sci. 2018 Jul 3;19(7):1952. doi: 10.3390/ijms19071952.
Human adipose-derived stromal cells (hASCs) are widely known for their immunomodulatory and anti-inflammatory properties. This study proposes a method to protect cells during and after their injection by encapsulation in a hydrogel using a droplet millifluidics technique. A biocompatible, self-hardening biomaterial composed of silanized-hydroxypropylmethylcellulose (Si-HPMC) hydrogel was used and dispersed in an oil continuous phase. Spherical particles with a mean diameter of 200 μm could be obtained in a reproducible manner. The viability of the encapsulated hASCs in the Si-HPMC particles was 70% after 14 days in vitro, confirming that the Si-HPMC particles supported the diffusion of nutrients, vitamins, and glucose essential for survival of the encapsulated hASCs. The combination of droplet millifluidics and biomaterials is therefore a very promising method for the development of new cellular microenvironments, with the potential for applications in biomedical engineering.
人脂肪来源的基质细胞（hASCs）因其免疫调节和抗炎特性而被广泛知晓。本研究提出了一种在使用液滴微流控技术包封在水凝胶中时保护细胞的方法。使用了一种由硅烷化羟丙基甲基纤维素（Si-HPMC）水凝胶组成的生物相容性、自硬化生物材料，并分散在连续油相中。以可重复的方式获得了平均直径为 200μm 的球形颗粒。在体外培养 14 天后，包封在 Si-HPMC 颗粒中的 hASCs 的存活率为 70%，证实了 Si-HPMC 颗粒支持了营养物质、维生素和葡萄糖的扩散，这些是包封的 hASCs 存活所必需的。因此，液滴微流控和生物材料的结合是开发新的细胞微环境的一种很有前途的方法，具有在生物医学工程中的应用潜力。
