Liu Yisi, Wang Meng, Luo Yixuan, Liang Qianyi, Yu Yin, Chen Fei, Yao Jun
Collaborative Innovation Center of Regenerative Medicine and Medical Biological Resources Development and Application, Guangxi Medical University, Nanning 530021, China.
Center for Materials Synthetic Biology, CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Gels. 2021 Dec 14;7(4):263. doi: 10.3390/gels7040263.
Stem cells hold tremendous promise for the treatment of cartilage repair in osteoarthritis. In addition to their multipotency, stem cells possess immunomodulatory effects that can alleviate inflammation and enhance cartilage repair. However, the widely clinical application of stem cell therapy to cartilage repair and osteoarthritis has proven difficult due to challenges in large-scale production, viability maintenance in pathological tissue site and limited therapeutic biological activity. This review aims to provide a perspective from hydrogel-focused approach to address few key challenges in stem cell-based therapy for cartilage repair and highlight recent progress in advanced hydrogels, particularly microgels and dynamic hydrogels systems for improving stem cell survival, retention and regulation of stem cell fate. Finally, progress in hydrogel-assisted gene delivery and genome editing approaches for the development of next generation of stem cell therapy for cartilage repair in osteoarthritis are highlighted.
干细胞在骨关节炎软骨修复治疗方面具有巨大潜力。除了具有多能性外，干细胞还具有免疫调节作用，可减轻炎症并促进软骨修复。然而，由于在大规模生产、病理组织部位的活力维持以及有限的治疗生物活性等方面存在挑战，干细胞疗法在软骨修复和骨关节炎中的广泛临床应用已被证明具有难度。本综述旨在从以水凝胶为重点的方法提供一个视角，以解决基于干细胞的软骨修复治疗中的几个关键挑战，并突出先进水凝胶，特别是微凝胶和动态水凝胶系统在改善干细胞存活、保留和干细胞命运调控方面的最新进展。最后，强调了水凝胶辅助基因递送和基因组编辑方法在开发用于骨关节炎软骨修复的下一代干细胞疗法方面的进展。
