纳米分辨率下分子马达的多色追踪
Multicolor Tracking of Molecular Motors at Nanometer Resolution.
作者信息
Wichner Sara M, Yildiz Ahmet
机构信息
Department of Chemistry, University of California, Berkeley, CA, USA.
Department of Physics, University of California, Berkeley, CA, USA.
出版信息
Methods Mol Biol. 2018;1805:139-149. doi: 10.1007/978-1-4939-8556-2_8.
Abstract
Molecular motors move processively along cytoskeletal filaments through stepping of their catalytic head domains. Observation of the stepping movement of the heads reveals the mechanism of motor processivity and how they coordinate the cycles of the catalytic heads during processive motility. This chapter will discuss recent developments in simultaneous observation of the stepping motions of the two heads using multicolor single particle tracking microscopy.
摘要
分子马达通过其催化头部结构域的步进沿着细胞骨架丝进行连续移动。对头部步进运动的观察揭示了马达连续性的机制以及它们在连续运动过程中如何协调催化头部的循环。本章将讨论使用多色单粒子跟踪显微镜同时观察两个头部步进运动的最新进展。
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