神经生长因子诱导PC12细胞的神经突生长涉及微管组装和组装促进因子的协同诱导。
Nerve growth factor-induced neurite outgrowth in PC12 cells involves the coordinate induction of microtubule assembly and assembly-promoting factors.
作者信息
Drubin D G, Feinstein S C, Shooter E M, Kirschner M W
出版信息
J Cell Biol. 1985 Nov;101(5 Pt 1):1799-807. doi: 10.1083/jcb.101.5.1799.
Abstract
Nerve growth factor (NGF) regulates the microtubule-dependent extension and maintenance of axons by some peripheral neurons. We show here that one effect of NGF is to promote microtubule assembly during neurite outgrowth in PC12 cells. Though NGF causes an increase in total tubulin levels, the formation of neurites and the assembly of microtubules follow a time course completely distinct from that of the tubulin induction. The increases in microtubule mass and neurite extension closely parallel 10- and 20-fold inductions of tau and MAP1, proteins shown previously to promote microtubule assembly in vitro. When NGF is removed from PC12 cells, neurites disappear, microtubule mass decreases, and both microtubule-associated proteins return to undifferentiated levels. These data suggest that the induction of tau and MAP1 in response to NGF promotes microtubule assembly and that these factors are therefore key regulators of neurite outgrowth.
摘要
神经生长因子（NGF）可调节某些外周神经元轴突依赖微管的延伸和维持。我们在此表明，NGF的一个作用是在PC12细胞的神经突生长过程中促进微管组装。尽管NGF会导致微管蛋白总量增加，但神经突的形成和微管的组装遵循的时间进程与微管蛋白诱导的时间进程完全不同。微管质量和神经突延伸的增加与tau和MAP1分别被诱导10倍和20倍密切平行，先前已表明这两种蛋白质在体外可促进微管组装。当从PC12细胞中去除NGF时，神经突消失，微管质量减少，并且两种微管相关蛋白都恢复到未分化水平。这些数据表明，响应NGF诱导的tau和MAP1促进了微管组装，因此这些因子是神经突生长的关键调节因子。
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