巴西亚马逊地区阿克里州一个流行区皮肤利什曼病的分子诊断
Molecular diagnosis of cutaneous leishmaniasis in an endemic area of Acre State in the Amazonian Region of Brazil.
作者信息
Araujo-Pereira Thais de, Pita-Pereira Daniela de, Moreira Regina Barbosa, Silva-Galdino Tainah, Duarte Márcia P de Oliveira, Brazil Reginaldo Peçanha, Britto Constança
机构信息
Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz/Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
Laboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz/Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
出版信息
Rev Soc Bras Med Trop. 2018 May-Jun;51(3):376-381. doi: 10.1590/0037-8682-0232-2017.
INTRODUCTION
This study proposes to identify the Leishmania species found in the skin lesions of cutaneous leishmaniasis (CL) patients from Brasiléia municipality (Acre).
METHODS
Skin biopsy imprints or biopsy fragments were assayed via kDNA-PCR/RFLP and FRET-real-time PCR.
RESULTS
Of individuals with suspected CL, 18 were positive for Leishmania kDNA. Leishmania (Viannia) braziliensis (61.1%) and Leishmania (Viannia) guyanensis (5.5%) were identified in the positive samples.
CONCLUSIONS
These results are congruent with the previous reports in Acre and Bolivia, revealing L. braziliensis as the most prevalent species. L. guyanensis identification also corroborates with the epidemiology of the disease in the Amazon Basin.
引言
本研究旨在鉴定来自阿克里州巴西利亚市皮肤利什曼病（CL）患者皮肤病变中发现的利什曼原虫种类。
方法
通过kDNA-PCR/RFLP和FRET实时PCR检测皮肤活检印记或活检片段。
结果
在疑似CL的个体中，18例利什曼原虫kDNA呈阳性。在阳性样本中鉴定出巴西利什曼原虫（维安尼亚亚种）（61.1%）和圭亚那利什曼原虫（维安尼亚亚种）（5.5%）。
结论
这些结果与阿克里州和玻利维亚之前的报告一致，表明巴西利什曼原虫是最常见的种类。圭亚那利什曼原虫的鉴定也与亚马逊盆地该疾病的流行病学情况相符。
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