Department of Surgery, Transplant & Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA.
Department of Biochemistry & Biophysics, Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA.
Nanomedicine (Lond). 2018 Jun;13(12):1417-1432. doi: 10.2217/nnm-2018-0011. Epub 2018 Jul 4.
The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D (1,25(OH)D) and augmenting human CAMP induction.
MATERIALS & METHODS: Nanofibrous wound dressings containing 1,25(OH)D were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo.
1,25(OH)D was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)D showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)D-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants.
1,25(OH)D containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.
本研究旨在开发一种基于纳米纤维的敷料，能够局部持续递送 1α，25-二羟维生素 D（1，25（OH）D）并增强人 CAMP 诱导。
通过静电纺丝成功制备了含有 1，25（OH）D 的纳米纤维状伤口敷料，并进行了体外、体内和离体研究。
1，25（OH）D 成功加载到纳米纤维中，包封效率大于 90%。1，25（OH）D 从纳米纤维中持续释放超过 4 周。用负载 1，25（OH）D 的聚（ε-己内酯）纳米纤维处理 U937 和 HaCaT 细胞，可显著诱导单核细胞和角质细胞、人源化转基因小鼠皮肤伤口和人皮肤标本人工伤口中 hCAP18/LL37 的表达。
含 1，25（OH）D 的纳米纤维敷料可通过诱导抗菌肽产生增强固有免疫。
