基于纳米纤维的缝线可诱导内源性抗菌肽。
Nanofiber-based sutures induce endogenous antimicrobial peptide.
机构信息
Department of Surgery-Transplant & Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA.
Chongqing Academy of Animal Sciences & Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Chongqing, China.
出版信息
Nanomedicine (Lond). 2017 Nov;12(21):2597-2609. doi: 10.2217/nnm-2017-0161. Epub 2017 Sep 29.
AIM
The aim of this study was to develop nanofiber-based sutures capable of inducing endogenous antimicrobial peptide production.
METHODS
We used co-axial electrospinning deposition and rolling to fabricate sutures containing pam3CSK4 peptide and 25-hydroxyvitamin D (25D).
RESULTS
The diameters and mechanical properties of the sutures were adjustable to meet the criteria of United States Pharmacopeia designation. 25D exhibited a sustained release from nanofiber sutures over 4 weeks. Pam3CSK4 peptide also showed an initial burst followed by a sustained release over 4 weeks. The co-delivery of 25D and pam3CSK4 peptide enhanced cathelicidin antimicrobial peptide production from U937 cells and keratinocytes compared with 25D delivery alone. In addition, the 25D/pam3CSK4 peptide co-loaded nanofiber sutures did not significantly influence proliferation of keratinocytes, fibroblasts, or the monocytic cell lines U937 and HL-60.
CONCLUSION
The use of 25D/pam3CSK4 peptide co-loaded nanofiber sutures could potentially induce endogenous antimicrobial peptide production and reduce surgical site infections.
目的
本研究旨在开发能够诱导内源性抗菌肽产生的基于纳米纤维的缝线。
方法
我们使用同轴电纺沉积和滚动技术来制备含有 Pam3CSK4 肽和 25-羟维生素 D（25D）的缝线。
结果
缝线的直径和机械性能可调节以满足美国药典的标准。25D 在 4 周内从纳米纤维缝线中持续释放。Pam3CSK4 肽也表现出初始突释，然后在 4 周内持续释放。与单独使用 25D 相比，25D 和 Pam3CSK4 肽的共递送增强了 U937 细胞和角质形成细胞中防御素抗菌肽的产生。此外，载有 25D/Pam3CSK4 肽的纳米纤维缝线对角质形成细胞、成纤维细胞以及单核细胞系 U937 和 HL-60 的增殖没有显著影响。
结论
使用载有 25D/Pam3CSK4 肽的纳米纤维缝线可能会诱导内源性抗菌肽的产生，并减少手术部位感染。
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