Krittanai Supaluk, Kitisripanya Tharita, Udomsin Orapin, Tanaka Hiroyuki, Sakamoto Seiichi, Juengwatanatrakul Thaweesak, Putalun Waraporn
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University, Khon Kaen University, Khon Kaen, Thailand.
Biomed Chromatogr. 2018 Nov;32(11):e4330. doi: 10.1002/bmc.4330. Epub 2018 Aug 15.
Pueraria candollei or White Kwao Krua (Leguminosae) is an indigenous plant in Thailand which has long been used in Thai traditional medicine. The tuberous root of this plant is widely used for rejuvenation, particularly in elder women. Among the bioactive compounds in P. candollei, miroestrol and puerarin exhibit estrogenic activity. This study aims to develop an immunochromatographic strip (ICS) with a colloidal gold-based detection system for the simultaneous detection of miroestrol and puerarin in a one-step analysis. The developed method is sensitive and specific for the detection of miroestrol and puerarin in raw materials and marketed products. The detection limits of miroestrol and puerarin were 0.15 and 4.5 μg, respectively. In addition, the results from the developed ICS were confirmed with an enzyme-linked immunosorbent assay and presented a good correlation between these two methods. This is the first report on the development of an ICS that can detect miroestrol and puerarin in one step. The developed ICS provides a simplified method for the detection of miroestrol and puerarin in P. candollei and Pueraria spp.
白花葛（豆科）是泰国的本土植物，长期以来一直用于泰国传统医学。这种植物的块根被广泛用于恢复活力，尤其是老年女性。在白花葛的生物活性化合物中，米罗雌醇和葛根素具有雌激素活性。本研究旨在开发一种基于胶体金检测系统的免疫层析试纸条（ICS），用于一步分析同时检测米罗雌醇和葛根素。所开发的方法对原材料和市售产品中米罗雌醇和葛根素的检测具有灵敏性和特异性。米罗雌醇和葛根素的检测限分别为0.15μg和4.5μg。此外，所开发的免疫层析试纸条的结果通过酶联免疫吸附测定得到证实，并且这两种方法之间呈现出良好的相关性。这是关于开发一种能够一步检测米罗雌醇和葛根素的免疫层析试纸条的首次报道。所开发的免疫层析试纸条为检测白花葛和葛属植物中的米罗雌醇和葛根素提供了一种简化方法。
