Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, 40002, Thailand.
J Nat Med. 2019 Jun;73(3):577-583. doi: 10.1007/s11418-019-01307-6. Epub 2019 Apr 11.
Pueraria candollei (P. candollei) is a traditional Thai herb widely used for estrogen replacement therapy because it contains many unique chromenes that possess potent estrogenic activity, one of which is known as isomiroestrol. Since isomiroestrol is a promising compound that is solely present in P. candollei, it can be used as an identifying marker for standardization of P. candollei. Here, we developed a lateral-flow immunochromatographic strip (ICS) test using a colloidal gold nanoparticle-conjugated anti-isomiroestrol monoclonal antibody (12C1-mAb) for the detection of isomiroestrol in plant samples and products of P. candollei. The advantages of the developed ICS over an enzyme-linked immunosorbent assay are its simplicity and rapidity, as the ICS test can be completed 15 min after dipping the strip into the analyte solution. The detectable concentration of isomiroestrol was 7.0 µg/mL. Considering the demand for the standardization of P. candollei due to concerns regarding its quality, our ICS test using isomiroestrol as an identifying marker would be effective and useful to assess the presence of isomiroestrol.
野葛（Pueraria candollei）是一种传统的泰国草药，被广泛用于雌激素替代疗法，因为它含有许多独特的色烯，具有很强的雌激素活性，其中一种被称为异米列醇。由于异米列醇是一种很有前途的化合物，仅存在于野葛中，因此可以用作野葛标准化的鉴定标记物。在这里，我们使用胶体金纳米粒子偶联的抗异米列醇单克隆抗体（12C1-mAb）开发了一种侧向流动免疫层析检测试剂盒（ICS），用于检测植物样品和野葛制品中的异米列醇。与酶联免疫吸附测定相比，开发的 ICS 具有简单快速的优点，因为将条带浸入分析物溶液 15 分钟后即可完成 ICS 测试。异米列醇的检测浓度为 7.0 µg/mL。考虑到对野葛标准化的需求，以及对其质量的关注，我们使用异米列醇作为鉴定标记物的 ICS 测试将有效且有助于评估异米列醇的存在。
