Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
J Nat Med. 2022 Jun;76(3):521-545. doi: 10.1007/s11418-022-01605-6. Epub 2022 Feb 16.
Phytoproducts are involved in various fields of industry. Small-molecule (Mw < 900 Da) organic compounds can be used to indicate the quality of plant samples in the perspective of efficacy by measuring the necessary secondary metabolites and in the perspective of safety by measuring the adulterant level of toxic compounds. The development of reliable detection methods for these compounds in such a complicated matrix is challenging. The lateral flow immunoassay (LFA) is one of the immunoassays well-known for its simplicity, portability, and rapidity. In this review, the general principle, components, format, and application of the LFA for phytoproducts are discussed.
植物制品应用于各个工业领域。小分子(Mw<900Da)有机化合物可以通过测量必要的次生代谢物来从功效的角度、通过测量有毒化合物的掺杂物水平来从安全性的角度,用于指示植物样本的质量。在这样复杂的基质中开发用于这些化合物的可靠检测方法具有挑战性。侧向流动免疫分析(LFA)是一种众所周知的免疫分析方法,因其简单、便携和快速而受到关注。本文综述了 LFA 用于植物制品的一般原理、组成部分、格式和应用。